药用植物次生代谢相关酶基因克隆方法综述

Review on Gene Cloning Methods Involved in Medicinal Plant Secondary Metabolism

目的综述药用植物次生代谢酶基因克隆可采用的方法策略,为其基因克隆提供参考。方法查阅相关文献进行总结和归纳。结果阐述了用于克隆药用植物次生代谢酶基因的功能克隆方法、表型克隆方法(DD-PCR、cDNA–AFLP、SSH)、转座子标签克隆方法及图位克隆方法。结论适用于药用植物次生代谢相关酶基因克隆的技术方法有很多种,研究者可根据研究基础、目的和实验条件等进行选择。

OBJECTIVE Reviewing gene cloning methods and strategies that can be used in medicinal plant secondary metabolism, to provide selection references. METHODS Searching and summarizing literatures on gene cloning of plants, especially of medicinal plants. RESULTS Gene cloning methods used in medicinal plant secondary metabolism, such as functional cloning method, phenotype cloning methods, transposon tagging and map-based cloning method were elaborated. CONCLUSION There are many methods used to clone genes in medicinal plant secondary metabolism, researchers should choose the suitable one with different basis, purposes and experiment condition.