HPLC测定氯碘羟喹的含量及有关物质

Determination of Clioquinol and Its Related Substances by HPLC

目的建立氯碘羟喹的含量及有关物质的HPLC测定方法。方法采用C8柱,以乙二胺四醋酸二钠缓冲液-甲醇(35∶65)为流动相;流速1.0mL.min-1;检测波长为254nm。结果氯碘羟喹的线性范围为3～60μg·mL-1(r=0.9999),平均回收率为99.8%,RSD为0.13%(n=9)。结论方法简便、准确,专属性强,可作为产品的含量和有关物质的检测。

OBJECTIVE To establish an HPLC method for content determination of clioquinol and related substances in clioquinol raw material. METHODS A C8 column was used with the mobile phase of the buffer solution-methanol （35 ： 65）. The flow rate was 1.0 mL.min^-1 and the detection wavelength was 254 nm. RESULTS The calibration curves of clioquinol was linear in the range of 3-60 pg.mL^-1（r=-0.999 9）, and the average recoveries was 99.8%, RSD was 0.13%（n=9）. CONCLUSION The method is rapid, accurate and reliable for determining the content and related substances in clioquinol raw material.