长波紫外线对M13mp2噬菌体的致突变作用

Mutagenesis of M13mp2 phage by near ultraviolet light.

以野生型大肠菌ＣＳＨ５０及ｍｕｔＭ缺陷的大肠菌ＭＦ６７为宿主，检测了长波紫外线（ＵＶＡ）对Ｍ１３ｍｐ２噬菌体单链ＤＮＡｌａｃＺα基因区域的致突变能力。结果显示，ＵＶＡ照射可引起噬菌体ｌａｃＺα基因区域的突变，在ｍｕｔＭ缺陷的宿主中，ＵＶＡ照射的致突变作用更明显。进一步研究了抗氧化剂甘露醇对ＵＶＡ照射致突变作用的影响，结果显示，０．１ｍｏｌ／Ｌ的甘露醇可以部分拮抗ＵＶＡ的致突变作用。说明ＵＶＡ照射具有致突变作用。

The mutagenic potential of UVA (ultraviolet A) on the lacZα region of M13mp2 phage was observed in wild type CSH50 and mutM deficient (MF67) E.coli .The results showed that UVA induced mutations at lacZα region of phage and the mutation frequency was significantly higher in MF67 than in CSH50. The effect of free radical scavanger (mannitol) on mutagenesis by UVA was also investigated. The mutagenesis was inhibited in part by 0.1mol/L of mannitol. These results indicate that UVA can cause mutation in phage and its mutagenic effect can be ascribed to oxidative damage of DNA.