无锡地区妇女宫颈HPV感染及基因型别分析

Analysis of cervix HPV infection and its genotypes in women in Wuxi area

目的对疑似人乳头瘤病毒(HPV)感染的970例妇女进行HPV基因分型检测,以探讨其临床意义。方法用专用宫颈刷采取妇女宫颈的脱落细胞,采用基因芯片法检测970例HPV临床样本,并进行基因分型。结果970例样本中,HPV感染390例,阳性率40.2%。共检出12种HPV亚型,检出感染单一HPV亚型260例(26.8%),感染两种亚型以上130例(13.4%)。低危型主要为HPV6、11,高危型主要为HPV16、33、52、58、18等。结论无锡地区妇女宫颈HPV感染率较高,感染亚型主要是6、11型。HPV基因分型检测对于生殖道感染以及子宫颈癌的早期发现以及预防和治疗具有重要意义。

Objective To detect the genotypes of human papillomaviral infection（HPV） in 970 women who were suspicious as HPV infection, and to investigate its clinical significance. Methods Cervical cast-off cells were acquired with the special cervix brush. DNA hybridization technique was applied to detecting HPV genotypes in 970 clinical specimens. Results Totally 390 case（40.2%） were HPV positive among 970 clinical specimens. A total of 12 subtypes were detected. In 390 cases of HPV infection,260 cases （26.8 %） were infected with single HPV subtype, and while 130 cases （ 13.4 %） were with infection of two or above HPV subtypes. HPV6 and 11 were the most frequent low-risk subtypes, and in the high-risk subtypes, HPV16,33,52,58 and 18 were dominant. Conclusion The cervix HPV infection rate is considerable in women in Wuxi area. HPV6 and 11 are the most frequent subtypes. The determination of HPV genotypes plays all important role in early discovery,prevention and treatment of gential tract and cervical cancer.