性病门诊患者解脲脲原体分离株的分子分群及血清分型研究

Study on biovar and serotype of Ureaplasma urealyticum isolates in out-patients with sexually transmitted disease

目的了解性病门诊患者解脲脲原体(Uu)分离株的血清分型与分子分群的分布状况与相互关系。方法采用液体加固体的方法从临床标本中分离获得解脲脲原体临床菌株,纯化后采用PCR扩增Uu多条带基因进行分子分群,采用代谢抑制试验进行血清分型。结果从377例性病门诊患者中分离到Uu 117例,阳性率31%。其中男性54例,阳性率46%;女性63例,阳性率54%。成功进行分子分群114例,其中生物1群47例,占41.2%;生物2群67例,占58.8%;进行血清分型60例,其中单个型别41例,占68.3%;多型别合并19例,占31.7%。出现频率高的型别为7型24例(29.63%),2型19例(23.46%),14型11例(13.58%)和4型7例(8.64%)。未见3、5、12、13 4种血清型别。16.7%Uu分离株(10/60)存在分型与分群结果不符情况。结论性病门诊患者Uu分离株中,生物2群所占比例多于生物1群,血清分型以7、2、14、4等型别常见,发现部分菌株分子分群与血清分型结果不符现象。

Objective To investigate the correlation and distribution of biovar and serotype of Ureaplasma urealyticum（Uu） isolates in out-patients with sexually transmitted disease（STD）. Methods The liquid culture and solid culture were applied to isolating Uu strains from the clinical specimens. After purification of Uu strains,the multiple banded antigen gene of Uu was amplified by using polymerase chain reaction（PCR）,and then the biovar of Uu isolates was performed. The serotype was determined with metabolic inhibition test. Results Totally 117 cases（31M） were Uu positive from 377 clinical specimens of STD out-patients, Among them, 54 cases（46 %） were isolated from males, and 63 cases（54%） were from females. A total of 114 isolates were successfully biovared, in which 47 cases （41.2%） were biovar 1,and 67 cases（58.8%） were biovar 2;60 isolates were serotyped,in which 41 cases（68.3 % ） were single serotype, and 19 cases（31.7 %） contained multiple serotypes. The most frequent serotypes were as follows ： serotype 7 （24 cases, 29.63 % ） ; serotype 2 （ 19 cases, 23.46 % ） ; serotype 14（11 cases,13.58%）;serotype 4（7 cases,8.64%）. No serotype 3,5,12,or 13 was detected. The discrepancy between biovar and serotype was found in 16.7%（10/60） of Uu isolates. Conclusion The main biovar of Uu isolates is biovar 1 in STD out-patients, and serotype 7,2,14 and 4 were dominant. The discrepancy between biovar and serotype exists in some Uu isolates.