MMP-9和TIMP-1在不明原因不孕症患者子宫内膜的表达

The expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in the endometrium of women with unexplained infertility

目的检测基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制物-1(TIMP-1)在不明原因不孕症子宫内膜和正常子宫内膜中的表达,探讨两者与不明原因不孕的关系。方法应用免疫组化SP法,检测MMP-9和TIMP-1在20例不孕症患者的种植窗期子宫内膜和20例正常子宫内膜中的表达。结果MMP-9/TIMP-1在2组子宫内膜的腺上皮细胞和基质细胞的胞浆中均有不同程度的表达。MMP-9/TIMP-1在正常子宫内膜中呈高表达状态;在不孕子宫内膜表达较低,与正常子宫内膜组织相比差异有统计学意义(P<0.05)。结论不明原因不孕症妇女子宫内膜种植窗期MMP-9和TIMP-1的低表达,可能是导致不明原因不孕症的重要因素之一。

Objective To study the expression situation of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-l（TIMP-1） in the endometrium of women with unexplained infertility and normal endometrium,and to explore the relationship between MMP-9/TIMP-1 expres- sion and unexplained infertility. Methods Immunohistoehemical assay （SP method） was employed to detect the expression of MMP-9/TIMP-1 in 20 cases of impaired endometrium（unexplained infertility group,endometrial plantation window phase）and 20 cases of normal endometrium （healthy control group）. Results There were different levels of MMP-9/TIMP-1 expression in kytoplasms of glandular epicytes and stromal cells of all endometrial samples. The expression of MMP-9/TIMP-1 was significantly weaker in unexplained infertility group than that in healthy control group（P〈0.05）. Conclusion Low expression of MMP-9/TIMP-1 in the endometrial plantation window phase may be one of important factors for unexplained infertility.