摘要
目的研究转化生长因子-β1(TGF-1)在大鼠心脏移植排斥反应中的表达。方法以对照组(n=40)和新赛斯平组(CsA组,n=40)建立大鼠心脏移植模型,观察供心存活时间,于移植术后第1、3、5、7、9、11、14天分别取供心组织各5例,半定量RT-PCR的方法动态检测供心中TGF-1的表达情况。结果供心存活时间分别为:对照组(8.6±1.5)d,CsA组(20.4±5.1)d,两组间有显著差异(P<0.01)。两组中TGF-1表达的时间变化趋势与供心存活时间有一致性,但在两组中的表达强度不同,对照组较CsA组弱,二者间有显著性差异(P<0.01)。结论TGF-1的产生和表达可能因CsA的干预而增加,CsA的干预明显延长了供心的存活时间。
Objective The expression of TGF -β1 in the grafts of the rats with acute rejection of heart transplantation were studied and the role of TGF - β1 in the acute rejection of heart transplantation in rats investigated. Methods Allografts were divided into 2 groups ： control group （ n = 40）, cyclosporin A - treated group （ n = 40）. Hearts from SD rats were transplanted into a cervical location in Wistar recipients and the survival time of the allografts was observed. The local expression of TGF - 1 was detected at day 1, 3, 5,7, 9, 11, 14 by reverse transcription polymerase chain reaction （ RT - PCT） respectively. Results The survival time of the allografts was （ 8.6 ± 1.5 ） days and （20. 4 ± 5.1 ） days （ P 〈 0. 01 ）. The levels of the TGF - β1 expression were up - regulated in the group B. The up -regulation of TGF - 1 was closely correlated with the survival of the grafts. Conclusion The expression and production of TGF - β1 is up - regulated with the administration of cyclosporin A, which is favorite to the heart graft survival.
出处
《医药论坛杂志》
2009年第19期16-17,共2页
Journal of Medical Forum
基金
豫教高[2004]239号项目资助
