改良型大鼠激素性股骨头坏死模型建立和相关评价

The Improved Model's Establishment and Evaluation of Rat Femoral Head Necrosis Induced by Glucocorticoid

目的建立疲劳状态下大鼠激素性股骨头坏死模型,为激素性股骨头坏死的实验研究提供可靠的动物模型。方法4月龄健康Wistar大鼠随机分成实验组与对照组。臀肌注射地塞米松磷酸钠(20mg/kg),每周1次,共8周。实验组,置于跑步机中,以1km/h速度匀速跑动;对照组,生理状态下正常负重。分别于2、4、6、8周处死动物,一侧股骨头行病理学检查,一侧行生物力学检查。结果建立了股骨头坏死模型,病理学上单位面积内骨小梁面积、骨小梁宽度、每高倍视野单位面积下空泡陷窝率,两组比较有明显统计学差异(P<0.05)。生物力学上实验组中各个时期骨小梁刚度低于同期对照组;最大形变、压缩能量吸收均高于同期对照组(P<0.05)。结论成功建立了疲劳状态下激素性股骨头坏死的动物模型,突出了机械负重在股骨头坏死中的作用,是研究激素性股骨头坏死的良好实验模型。

Objective To create the rat models of the femoral head necrosis induced by glucocorticoid in tired condition. To afford reliable animal model for the experimental study of femoral head necrosis induced by glucocorticoid. Methods 40 healthy 4-month wistar rat were divided into two groups randomly,20 of models and 20 of control. After the buttocks injection of dexamethasone 20 mg/kg weakly for 8 weeks ,the model group were made to run on the running machine at a speed of lkm/h for 30 minutes twice a week ;and at the same time,the control group were not ,only with the physiological weight. Both groups were killed at a time interval of 2w,4w, 6w,8w. One leg was taken pathological examination,then the other side was taken biomechanical test. Results To creat the model of femoral head necrosis. In pathology there was statistical differences between the control and the model on the width of trabeculae, the area percent of trabeeulne and the count of empty osteocyte lacuna（P〈0.05）. In biomechanical test it was statisti cally lower than the control on the stiffness ,but higher on the deflection and energy absorbed. The osteoporosis is also getting serious. Conclusion To successfully creat rat models of the femoral head necrosis induced by glucocorticoid in tired condition. It was well experimental model to research femoral head necrosis induced by glucocorticoid.