急进高原外周血低氧应激肽及一氧化氮含量变化及其意义研究

Study on change of hypoxia stress peptide and nitrogen monoxidum in peripheral blood from the entering mountain rapidly man and its significance

目的分析健康成年人急进高原后血清低氧应激肽和血浆一氧化氮(NO)含量的变化,探讨其在高原低氧适应中的意义。方法应用比色法检测106例急进高原健康者、105例世居高原健康者、105例移居高原健康者和104例平原健康者对照血清低氧应激肽含量;应用亚硝酸盐/硝酸盐(NO2-/NO3-)试剂检测血浆NO2-/NO3-浓度以代表血浆NO含量。对于急进高原者,分别于急进高原后24 h、48 h、76 h、1周和1月检测血清低氧应激肽。结果急进高原组血清低氧应激肽含量明显高于平原对照组及世居高原组和移居高原组(P均<0.05),其余3组之间差异无统计学意义(P>0.05)。急进高原后24 h,血清低氧应激肽急剧增高并达峰值,48 h至76 h略有下降,但仍维持较高水平,1周至1月逐渐下降,但仍明显高于平原对照组。急进高原组血浆NO2-/NO3-浓度明显高于平原对照组及世居高原组和移居高原组(P均<0.05),世居高原组和移居高原组略低于平原对照组,但差异无统计学意义(P>0.05)。结论健康成人急进高原后,血清低氧应激肽和血浆NO水平会有明显增高,以适应突发的高原应激,并随着进入高原后时间的延长而逐渐恢复正常。

Objective To analyze the concentration changes of serum hypoxia stress peptide （HSP） and plasma nitrogen monoxidum （NO） in the entering mountain rapidly man and to explore the importance of HSP and NO in adaptation of man to altitude hypoxia. Methods The levels of serum HSP and plasma NO2-/NO3-were measured from 106 entering mountain rapidly men, 105 altitude in habitant,105 ahitude immigrant and 104 plain healthy controls, respectively by chromatometry and NO2-/NO3-reagent. Furthermore,the content of serum HSP was determined in 106 entering mountain rapidly men,respectively at the time of 24 h,48 h,76 h,1 week and 1 month after entering mountain. Results The level of serum HSP was significantly higher in entering mountain rapidly men than inhabitant,immigrant and plain controls （P〈0.05）, while there was no statistical difference between inhabitant,immigrant and plain controls （P〉0. 05）. At 24h after entering mountain rapidly, the level of serum HSP was significantly increased and reached the peak. At 48h and 76h, it was slightly decreased, but still maintained a high level. At 1 week and 1 month,it gradually and significantly went down, but was still significantly higher than that in plain controls. Similarly, the level of plasma NO2-/ NO3-was significantly elevated in entering mountain rapidly man, compared with inhabitant, immigrant and plain controls （P〈0.05）. It was lower in inhabitants and immigrants than plain controls, but the difference was not statistically significant （P〉0.05）. Conclusion The present results demonstrated that the levels of serum HSP and plasma NO were significantly up-regulated in healthy adults after entering mountain rapidly in order to adapt to altitude stress,and restored gradually to the normal level as time went by.