哮喘缓解期患者外周血白细胞介素-5的表达及牛磺酸的影响

The change of interleukin-5 in peripheral blood from asthma patients during paracmasis and intervention of taurine

目的观察哮喘缓解期患者在牛磺酸治疗前后外周血白细胞介素-5(IL-5)的水平及其mRNA的表达,探讨其临床意义。方法采用随机、对照、双盲、前瞻性研究方法,对84例轻、中度哮喘患者,从病情进入缓解期开始口服牛磺酸(A组)和安慰剂(B组),疗程1年,以门诊健康体检的10例患者为基础对照(C组),比较治疗前后哮喘症状的改善以及外周血IL-5及其mRNA的变化。结果治疗后的哮喘症状积分和肺功能A组与B组(12.5±4.8vs15.2±5.1分/月;FEV1,2.6±0.5vs2.2±0.8L)差异显著(P<0.05);A、B两组血清治疗前IL-5及其mRNA初始表达均显著高于C组,治疗后A、B两组血清IL-5的水平差异显著(16.3±4.5vs26.9±12.7 pg/mL,P<0.05);IL-5及其mRNA表达差异显著(0.21±0.09vs0.35±0.14,P<0.05);A组患者外周血IL-5及其mRNA表达与哮喘的症状积分呈显著正相关(r=0.42,P<0.05)。结论IL-5在哮喘缓解期仍存在过度表达且反映了哮喘病情的严重程度,可以为临床治疗提供良好的参考。

Objective To investigate the change of interleukin-5（IL-5） level and mRNA expression in peripheral blood from asthma patients during paracmasis before and after administration of taurine,and explore its clinical significance. Methods A randomized, placebo control, double blind and prospective study was conduced in 84 patients with mild to moderate asthma During paracmasis. The patients were randomly divided into group A（taurine taken orally for one year from beginning of paracmasis） and group B（placebo taken orally for one year from beginning of paracmasis）. The symptom score of asthma,lung function paramcter（FEVI）,IL-5 level and mRNA expression in peripheral blood were measured and recorded before and after treatment. The results were compared with those of healthy control group（group C,n= 10）. Results There were significant differences in the above indices between group A and group B：symptom score of asthma（12.5±4.8 vs 15.2±5.1） ;lung function parameter[FEV1 （L） 2.6 ± 0.5 vs 2.2 ± 0.8] ; IL-5 level（pg/mL） （ 16.3 ±4.5 vs 26.9± 12.7 ） ; IL-5 mRNA（0.21±0.09 vs 0.35 ±0.14）. The basic level and mRNA expression of IL-5 were significantly higher in group A and group B than that in group C. The serum level and mRNA expression of IL-5 were positively correlative with symptom score of asthma in group A （r=0.42, P〈0.05）. Conclusion The exists ovcrexpression of IL-5 in asthma patients during paraemasis and IL-5 level reflects the sc verity of pathogenetic condition. Therefore,IL-5 measurement may provide a good refcrence for clinical therapy of asthma.