金山绣线菊(Spiraea×bumalda ‘Gold Mound’)再生体系的建立

Establishment of Plantlet Regeneration System for Spiraea×bumalda 'Gold Mound'

以金山绣线菊为研究试材,以茎段为外植体,通过使用不同的生长调节剂、不同质量浓度配比对外植体腋芽萌发和增殖影响进行了研究,建立了金山绣线菊无菌培养系;同时,探讨了叶片作为外植体的再生体系的建立。研究结果表明:金山绣线菊的茎段在添加2.0mg·L-16-BA、0.2mg·L-1NAA的MS培养基上腋芽能迅速萌发且长势良好,萌发率为93.3%;叶片在添加2.0mg·L-12,4-D、0.4mg·L-16-BA、0.04mg·L-1IBA的MS培养基上培养20d后产生的愈伤组织,转接到去除2,4-D,添加0.8mg·L-16-BA、0.04mg·L-1NAA的MS培养基上继续培养50d后,不定芽分化率为90%,每个外植体平均再生不定芽数为3.5个;再生植株在添加0.5mg·L-1NAA、0.1%AC(活性炭)的1/2MS培养基上,生根率达到100%,生根苗移植于混合基质中,成活率为86.7%。

The effects of different plant growth regulators and concentrations on the mieropropagation and regeneration of Spiraea × bumalda ＇ Gold Mound＇ were studied. A plantlet regeneration system was established using stem sections as explants. Results showed that the axillary buds germinated and grew well on MS medium containing 2.0 mg·L^-1 6 - BA and 0.2 mg·L^-1 NAA, with a germination rate of 93.3 percent. The induced callus cultured on MS supplemented with 2.0 mg ·L^-1 2,4 - D, 0.4 mg·L^-1 6 - BA, and 0.04 mg·L^-1 IBA for 20 days were transferred into MS without 2,4-D supplemented with 0.8 mg·L^-1 6 - BA and 0.04 mg ·L^-1 NAA. After 50 days of culture, the differentiation rate of the adventitious buds was 90 percent and the number of adventitious buds per explant was 3.5. 1/2MS medium containing 0.5 mg·L^-1 NAA and 0.1 percent of active carbon was suitable for rooting, with a rooting rate of 100 percent. The survival rate of the transplanted plantlets reached 86.7 percent.