PTEN基因和FHIT基因在膀胱尿路上皮癌中的表达和意义

Expression and significance of PTEN and FHIT in bladder urothelial carcinoma

目的探讨磷酸酶和张力蛋白同源(phosphatase and tensin homolog,PTEN)基因及脆性组氨酸三联体(fragile histindine triad,FHIT)基因在膀胱尿路上皮癌组织中的表达水平及临床意义。方法采用免疫组织化学S-P法检测14例正常膀胱黏膜及37例膀胱尿路上皮癌组织中的PTEN及FHIT基因的表达水平。结果在14例正常膀胱组织中PTEN与FHIT基因均表达阳性或强阳性。在37例膀胱尿路上皮癌组织中PTEN基因阳性表达率为56.8%(21/37),其中阳性9例,强阳性12例,FHIT基因阳性表达率为48.6%(18/37),其中阳性8例,强阳性10例。随着肿瘤分期、分级的增加PTEN和FHIT基因表达均显著减少;PTEN基因的表达与FHIT基因的表达呈正相关(P<0.01)。结论PTEN与FHIT基因可能成为估计膀胱尿路上皮癌恶性程度及肿瘤侵袭性的一项有用的指标,联合检测PTEN和FHIT基因在膀胱尿路上皮癌中的表达,有助于对膀胱肿瘤的细胞分化程度及诊断做出正确评价。

Objective To investigate the expression of PTEN and FHIT in bladder urothelial carcinoma and their clinical significance. Methods Immunohistochemistry（S-P） were used to detect the expression of PTEN and FHIT in the 14 cases of normal bladder tissues and 37 cases of bladder urothelial carcinoma. Results The expression of PTEN and FHIT was positive or strong positive in 14 normal bladder tissues.The positive rate of expression of PTEN in 37 bladder urothelial carcinoma tissue were 56.8%,9 positive and 12 strong positive.The positive rate of expression of FHIT were 48.6%,8 positive and 10 strong positive.With tumor stage and grade increasing,the expression of PTEN and FHIT were significantly reduced.An obverse correlation was found between PTEN and FHIT expression in bladder urothelial carcinoma（P〈0.01）. Conclusions PTEN and FHIT may be a useful indicator to estimate the bladder urothelial carcinoma degree of malignancy and invasiveness.The detection of expression of PTEN and FHIT in the bladder urothelial carcinoma may be helpful to evaluate the degree of differentiation and diagnosis.