鞘内注射细胞周期素依赖性激酶-5抑制剂Roscovitine对慢性背根节压迫大鼠痛行为学的影响

The efects of intrathecal administration of cyclin-dependent kinase 5 inhibitor roscovitine on the pain behavior of CCD rats

目的探讨鞘内注射细胞周期素依赖性激酶-5（Cdk5）抑制剂Roscovitine对慢性背根节压迫（CCD）大鼠痛行为学的影响。方法雄性SD大鼠24只随机分为4组，每组6只：假手术组＋50％二甲基亚砜（S组）；CCD＋50％二甲基亚砜（C组）；CCD＋Roscovitine 50μg（R组）；CCD0组（C0组）C0组不注入任何药物，只作为对照。各组大鼠在CCD或假手术后第7天分别鞘内注射容积为10μl的50％DMSO或Roscovitine 50μg，分别观察给药后的1、4、8h大鼠机械缩爪阈值（paw withdrawal mechani-cal threshold，PwMT）和热缩爪潜伏期（paw withdrawal thermal latency，PWTL）。结果与基础值相比，除S组外，各组大鼠从术后开始PWMT和PWTL均明显降低（P〈0．05），R组给药后与C组相比PWMT在各时间点无明显改变，PWTL相比明显增加，且保持在较高水平（P〈0．05）。结论脊髓背角Cdk5参与神经病理性疼痛的信号转导过程。

Objective To study the effects of intrathecal injection of cyclin-dependent kinase 5 inhibitor Roscovitine on the pain behavior in chronic compression of dosal root ganglia （CCD） rats. Methods 24 male SD rats with intrathecal catheter properly inserted, were randomly divided into four groups（n=6）： sham operation group（S group）, 50%DMSO was used as the vehicle control for the intrathecal injection; CCD group treated with 50% DMSO （operated for chronic compression of right dorsal root ganglion, C group）; Roscovitine group （Roscovitine 50 μg,R group） and CCD0 group was not injected any drugs （C0 group）, just as that in control. To evaluate the behavioral changes,paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were measured 0, 7 days and 1, 4, 8 h after injection on the 7th day of CCD. Results Compared with the value-based, with the exception of S group, all of the rats after CCD PWMT and PWTL were significantly lower （P〈0.05）. PWMT of R group was no significant change at all times point. Compared to value-based PWTL of R group was a marked increase, and remained at a relatively high level （P〈0.05）. Conclusion Cdk5 involves in spinal dorsal horn of neuropathic pain signal transduction process.