肺癌新鲜组织印片p53蛋白表达的免疫组化检测

A Study on p53 Protein Expression in Touch Preperations of the Lung Cancer by Immunohistochemistry Method

为便于临床应用肺癌ｐ５３蛋白表达免疫组化检测方法，对３３例手术切除肺癌新鲜组织，分别制备组织印片及冰冻切片标本，用免疫组化ＡＢＣ法检测ｐ５３蛋白表达。结果：组织印片和冰冻切片的ｐ５３蛋白表达阳性率分别为６０．６０％和６３．６４％，２种方法的相关非常显著（Ｐ＜０．００１），检测结果差异不显著（Ｐ＞０．０５）。结果提示组织印片法检测ｐ５３蛋白有无异常表达简便、准确、可靠，不需做病理切片，有一定的临床应用前景。

To make the method searching the p53 expression of lung cancer usable wildely in clinic units , the author studied the p53 protein expression of 33 cases lung cancer resected surgically both with the fresh tissue touch preparations and with frozen tissue specimens by using immunohistochemistry ABC method. The results showed that the p53 positive rate of touch preparations and frozen tissue specimens was 60.60% and 63.64%, respectively. The statistic analysis to the results of two groups displayed very much remarkable relativity ( P <0.001), but the no significant discrepancy ( P >0.05). The conclusion is that the method of the touch preparations specimens has the convenient, accurate and reliable characteristis for testing p53 protein expression and needs not to do the common pathological specimens. There is some prospects for clinic use in the future.