人甲状旁腺素(1-34)对新生Wistar大鼠成骨细胞增殖作用的影响

Effect of Parathyroid Hormone on Proliferation of Osteoblast in Neonatal Wistar Rat

目的：研究外源性人甲状旁腺素（１３４）（ｈＰＴＨ（１３４））对原代培养Ｗｉｓｔａｒ新生鼠成骨细胞增殖作用的影响及蛋白激酶Ａ（ＰＫＡ）、蛋白激酶Ｃ（ＰＫＣ）在此过程中作用。方法：Ｗｉｓｔａｒ大鼠成骨细胞原代培养，３ＨＴｄＲ掺入法测细胞增殖。结果：１０８ｍｏｌ／ＬｈＰＴＨ（１３４）在以２４ｈ为一循环的前１２ｈ给药循环３次时３ＨＴｄＲ掺入较对照组增加３２％，而１０７ｍｏｌ／ＬｈＰＴＨ（１３４）对３ＨＴｄＲ掺入无影响。ｈＰＴＨ（１３４）７２ｈ持续作用组３ＨＴｄＲ掺入与对照组相比无明显差异。加入ＰＫＡ抑制剂后阻断ｈＰＴＨ（１３４）引起３ＨＴｄＲ掺入增加，加入ＰＫＣ抑制剂后部分阻断１０８ｍｏｌ／ＬｈＰＴＨ（１３４）引起３ＨＴｄＲ掺入增加。ｈＰＴＨ（１３４）在１０１１～１０８ｍｏｌ／Ｌ范围内刺激细胞增殖与浓度成正相关。结论：ＰＴＨ刺激成骨细胞增殖与给药方式及药物浓度有关，低浓度间歇性给与ＰＴＨ能刺激成骨细胞增殖。

To investigate the effect of Parathyroid hormone (PTH) on proliferation of osteoblasts and the effect of protein kinase A (PKA) and protein kinase C (PKC) in this process, we isolate the osteoblasts from newborn Wistar rat and count 3H thymidine incorporation radioactivity. The result indicated that 10 9 、10 8 mol/L hPTH(1 34) significantly increase the 3H thymidine incorporation for the first 12h of each 24h incubation cycle for 3 cycles (P<0.01). 10 7 mol/L hPTH (1 34) treated the cells by this manner had no effect compared with the control (P>0.05). While given the osteoblasts with 10 8 mol/L hPTH (1 34) for 72h continuously, the 3H thymidine incorporation had no discrepancy compared with the control. PKA inhibitor blocked the effect of PTH on osteoblasts, while PCK inhibitor partially block this process. From 10 11 to 10 8 mol/L, the effect of PTH on osteoblast′s proliferation is positively relative to its concentration. So we could say, PTH has diverse effect on osteoblasts proliferation depending on the exposure time and its concentration in vitro, and PKA signal transduction systems had the major effect in this process.