Experimental Study on the Influence of Electroacupuncture on Cardiac Sympathetic Nerve Activity and Cardiac Function in Acute Myocardial Ischemia Rabbits

目的:观察电针不同原穴对急性心肌缺血家兔心交感神经电活动及心功能的影响,了解心交感神经在针刺调整急性心肌缺血过程中的作用机制。方法:健康青紫蓝家兔40只,随机选8只作正常组,其余家兔通过静脉注射垂体后叶素复制急性心肌缺血模型,将模型复制成功的家兔随机分为模型组、电针"神门"组、电针"太溪"组和电针非经穴组。采用BIOPAC生物信号采集系统记录心交感神经电信号和心功能指标的变化,同时接入SKY-A8生物信号处理系统进行数据处理。结果:急性心肌缺血模型复制后,家兔的左心室收缩压力峰值(LVSP)及室内压上升段最大变化率(+dp/dt max)明显下降,室内压下降段最大变化率(-dp/dtmax)升高。在停针即刻,电针"神门"组家兔的+dp/dtmax、-dp/dt max及LVSP与模型组、电针"太溪"组及电针非经穴组相比较差异有统计学意义(P<0.05)。急性心肌缺血模型复制后家兔心交感神经电活动的频率一过性增多后急剧减少,与正常组比较差异有统计学意义(P<0.05)。在停针即刻,电针"神门"组家兔的心交感神经电活动的频率与模型组、电针"太溪"组及电针非经穴组家兔差异均有统计学意义(P<0.05),与正常组比较,差异性无统计学意义。结论:电针"神门"穴可明显改善急性心肌缺血家兔的心功能及心交感神经电活动,促进机体心脏功能的恢复;电针不同原穴对急性心肌缺血家兔的心功能及心交感神经电活动的影响存在相对特异性,其中以"神门"穴的调整作用较为明显,而"太溪"穴及非经穴的作用不明显;电针相关经穴能调整心交感神经的活动,从而实现抗心肌缺血的效应。交感神经是针刺抗心肌缺血的主要外周传出途径之一。

Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AMI）, and to understand the interaction mechanisms of cardiac sympathetic nerve in adjusting acute myocardial ischemia. Method： A total of forty healthy rabbits were selected and 8 were randomly chosen as normal control. The rest 32 rabbits were inducted acute myocardial ischemia （AMI） models by intravenous Pituitrin, then the AMI model rabbits were randomly divided into 4 groups, 8 as model group, and 8 as EA on Shenmen （HT 7） group, 8 as EA on Taixi （K/3） group, and 8 as EA on nonpoint group. BIOPAC collection system of biological signal was used to record the changes of CSNA and cardiac function, and the data was analyzed using a SKY-A8 disposal system. Result： The lefl ventricular systolic peak pressure （LVSP）, maximum rate of rise of LVSP （＋dp/dt max） descended obviously, and maximum rate of descent of LVSP （-dp/dt max） ascended after induction of AMI. The ＋dp/dt max, -dp/dt max and LVSP of EA on Shenmen （HT 7） group are significantly higher than the untreated AMI group, EA on Taixi （KI 3） group and EA on nonpoint group after EA. The frequency of cardiac sympathetic nerve activity increased significantly after induction of AMI, and then decreased sharply （compared with that of normal group, P〈0.05）. Following EA, the cardiac sympathetic nerve activity of EA on Shenmen （HT 7） group are significantly higher than the model group, EA on Taixi （KI 3） group and EA on nonpoint group. And they have no significant difference with normal group. Conclusion： EA on Shenmen （HT 7） could improve the cardiac function and cardiac sympathetic nerve activity of rabbits with AMI, and promote the restoration of cardiac function. There is specificity in the effects of EA on different Yuan-Primary acupoints with the best effect on Shenmen （HT 7）, and no effect on Taixi （KI 3） and nonpoint. EA on related acupoint can adjust the cardiac sympathetic nerve activity, and may moderate the effects of myocardial ischemia. The sympathetic nerve is one of the key periphery ways in using acupuncture against myocardial ischemia.