摘要
采用不同浓度硫酸锰分别处理大蒜根尖,通过常规染色体压片技术,观察大蒜根尖细胞有丝分裂。结果表明,随着处理液中硫酸锰浓度的升高(1.0、2.0、3.0、4.0、5.0 mmol/L),有丝分裂指数呈现M形曲线变化;随着处理时间的延长(12、24、48h),有丝分裂指数逐渐下降,说明硫酸锰具有细胞分裂抑制剂的作用,不同浓度的硫酸锰在不同处理时间内均能诱使大蒜根尖细胞发生染色体畸变,表现为染色体断裂、染色体桥、微核等。
The garlic root tips were treated with different concentrations solutions of MnSO4 （1.0, 2.0, 3. 0, 4.0, 5. 0 mmol/L） lasting different time（12,24,48h）, and then observed the mitosis with normal chromosome pressing technology. The results showed that with the increase of the concentration of MnSO4, the mitotic index presented an M-shape curve; with the lengthening of treatment time, the mitotic index decreased gradually,which explained that MnSO4 might he the mitotic inhibitor. Furthermore, the phenomena of chromosomal aberration such as chromosome breakage, chromosome bridge and mieronucleus were also observed during mitosis.
出处
《河南农业科学》
CSCD
北大核心
2009年第10期119-121,共3页
Journal of Henan Agricultural Sciences
关键词
硫酸锰
大蒜
有丝分裂指数
染色体畸变
Allium satimum L.
MnSO4
Mitotic index
Chromosomal aberration
