加兰他敏的提取和制备工艺研究

Study on the Process of Extracting and Preparing Galanthamine

以黄花石蒜(Lycoris aurea)鳞茎为材料,采用热回流法提取石蒜鳞茎中的加兰他敏,正交试验分析不同因素及水平对加兰他敏提取率的影响,采用一步碱化母液用不同溶剂萃取和高效液相色谱(HPLC)方法分离制备.结果显示:在料液比1∶10,95%乙醇,85℃下提取2次,每次2 h,加兰他敏的提取率可达81.12%,碱化萃取率可达77%,HPLC可制备纯度为92.52%的加兰他敏,其收率为50.8%.

Objective： To develop a new technology of extracting galanthamine. Methods： Galanthamine was extracted from the bulb of Lycoris aurea by heat reflux. An orthogonal experiment was conducted to analyze the influences of different factors and their levels on the extracting rate of galanthamine. One-step extraction by basification was adopted and HPLC was made for separation and preparation. Results： The optimal extract conditions were as follows： solid and liquid ratio was 1 ： 10, ethanol 95% ,temperature 85℃, extract 2 times for 2 h for each, under which the extraction rate of galanthamine was up to 81.12%. The yield of alkalization extraction and HPLC preparation was 77% and 50.8%, respectively. The purity of galanthamine was up to 92.5%. Conclusion. The process of alkalization extraction is simple and HPLC preparation can separate galanthamine, which is flexible to operate.