循环高速逆流色谱从银杏叶提取物中制备异鼠李素和山奈酚

Preparation of Kaempferol and Isorhamnetin from Ginkgo Leaves by Recycling High-Speed Counter-Current Chromatography

为研究分离异鼠李素和山奈酚的方法,制备两者纯品.银杏叶经乙醇提取、氯仿萃取、D101大孔吸附树脂纯化后得到银杏黄酮粗提物,粗提物再经循环高速逆流色谱(循环HSCCC)分离结构类似的异鼠李素和山奈酚,溶剂体系为正己烷∶乙酸乙酯∶甲醇∶水(5∶5∶5∶5).结果表明:粗提取经循环HSCCC循环3次后,异鼠李素和山奈酚达到很好的分离,得到了两者的单体,纯度均在97%以上.

Objective： To establish a good procedure for separating kaempferol and isorhamnetin and to gain the pure sample of the two compounds. Methods： Ginkgo （Ginkgo biloba） leaves were extracted by alcohol and then purified by chloroform extraction and D101 macroporous resin to obtain the crude extract of flavone. Then the recycling high-speed counter-current chromatography （recycling HSCCC） was used to separate kaempferol and isorhamnetin from the crude extract with a solvent system composed of n-hexaneethyl acetate-methanol-water （5 ： 5 ： 5 ： 5, v/v/v/v）. Results： Kaempferol and isorhamnetin achieved high resolution by recycling HSCCC preparation with three cycles and the purity of the two compounds prepared by recycling HSCCC were over 97 ~. Conclusion： The recycling HSCCC was capable of efficiently separating compounds similar in chemical constitution, which provides a convenient and rapid way to separate kaempferol and isorhamnetin from ginkgo leaves.