溃疡性结肠炎患者血清IL-1β、TNF-α和IL-10的表达及其意义

The expression and significance of IL-1β and TNF-α and IL-10 in the serum of patients with ulcerative colitis

目的通过对溃疡性结肠炎(UC)患者血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-10(IL-10)表达水平的检测,探讨它们在UC发生、发展过程中的作用。方法实验组分为UC组(60例)和正常对照组(30例)。IL-1β、TNF-α和IL-10表达水平的检测:应用放射免疫法(RIA)检测三者在UC组、正常对照组血清中的表达水平。结果UC组血清中IL-1β、TNF-α表达水平与正常对照组相比明显增高(P<0.01),并与临床上病情的严重程度相一致。UC组血清中IL-10的含量与正常对照组间的差异没有统计学意义(P>0.05)。结论UC发病过程中,促炎性细胞因子IL-1β、TNF-α与抑炎性细胞因子IL-10的表达水平不一致,可能存在细胞因子网络的平衡失调。

Objective To detect the expressions of interleukin-1β（IL-1β）, tumor necrosis factor α（TNF-α）and interleukin-10（IL-10） in the serum of human ulcerative colitis（UC） ,and to investigate the role of those in the progression of ulcerative colitis. Methods Experiment group： UC group（60 cases） and normal control（30 cases）. The expressions of IL-1β, TNF-α and IL-10 in the serum of UC group and normal control were detected by radioimmunoassay（RIA）. Results The serum levels of IL-1β and TNF-α were significantly higher in UC group than those in normal control（P〈0.01） , and these changes were corre-sponding to the clinical symptoms. But there was no statistical significance in the serum level of IL-10 between UC group and normal control（P〉0.05）. Conclusion The disbalanee of cytokines may play an important role in the pathogenesis of UC.