bcl-2与NF—κB／p65在弥漫性大B细胞淋巴瘤不同亚型中表达的意义

Expressions of bcl-2 and NF-κB/p65 in distinct subgroups of diffuse large B-cell lymphoma and their clinical significance

目的探讨bcl-2与NF—κB／p65蛋白在弥漫性大B细胞淋巴瘤（DLBCL）不同亚型中的表达及其意义。方法用免疫组织化学方法检测DLBCL患者CD10、bcl-6、MUM-1蛋白的表达，以Hans等的分型原则将其划分为GCB和非GCB／ABC亚型，同时标记bcl-2与NF—κB／p65抗体，比较bcl-2与NF—κB／p65蛋白在GCB和ABC亚型中的表达情况并分析二者与DLBCL两种主要亚型生存率的相关性。结果bcl-2与NF—κB／p65蛋白在DLBCL中的表达率为67．1％和77．1％，二者表达呈正相关；GCB亚型中bcl-2与NF—κB／p65的表达率分别为52．0％和56．0％，ABC亚型中bcl-2与NF—κB／p65蛋白的表达率分别为75．6％和88．9％，ABC亚型中bcl-2与NF—κB／p65的表达率高于GCB亚型；在GCB亚型中，bcl-2与NF—κB／p65蛋白的表达与总生存率无显著的相关性，而在ABC亚型DLBCL中，bcl-2与NF—κB／p65蛋白的表达与生存率密切相关。结论仅在ABC亚型DLBCL中，bcl-2与NF—κB／p65蛋白的表达是影响预后的重要不利因素，因此，需在DLBCL亚分型的基础上评估bcl-2与NF—κB／p65蛋白表达的预后意义。

Objective To investigate the expression and clinical significance of bcl-2 and NF-κB/ p65 in different subgroups of diffuse large B-cell lymphoma （DLBCL）. Methods Immunohistochemical staining was used to detect the expressions of CD10, bcl-6, and MUM-1 in the DLBCL patients. According to immunohistochemical algorithm of Hans et al. DLBCL were subdivided into GCB and non-GCB/ABC subgroups and the expressions of bcl-2 and NF-κB/p65 were detected. The expressions of bcl-2 and NF-κB/ p65 in GCB DLBCL were compared with that in ABC DLBCL, and the correlation of bcl-2 and NF-κB/p65 expressions with survival in the two major subgroups of DLBCL were analyzed. Results The expression rates of bcl-2 and NF-κB/p65 proteins in DLBCL were 67.1% and 77.1%, and there was significant correlation between them. The expression rates of bcl-2 and NF-κB/p65 were 52.0 % and 56.0 % in GCB DLBCL, but were 75.6 % and 88.9 % in ABC DLBCL. The expression rates of two proteins were higher in ABC DLBCL than in GCB DLBCL. There was no significant correlation between bcl-2 and NF-κB/p65 protein expressions and overall survival within the GCB DLBCL subgroup, but bc1-2 and NF-κB/p65 expressions had a significant effect on overall survival within the ABC subgroup. Conclusion bcl-2 and NF-κB/p65 expressions are associated with poor survival in the ABC subgroup only. Hence, the significance of bcl-2 and NF-κB/p65 protein expressions should be assessed in the context of DLBCL subgroups in the future.