组蛋白乙酰化、甲基化调控异常在弥漫性大B细胞淋巴瘤中的意义

Study on irregulatory modification of histone acetylation, methylation in diffuse large B-cell lymphoma

目的研究组蛋白H3（H3K9、H3K14）、H4（H4K5、H4K8、H4K12、H4K16）乙酰化、组蛋白H3K4、H3K9甲基化水平在弥漫性大B细胞淋巴瘤（DLBCL）细胞的表达意义及其相关性。方法采用免疫组织化学sP法检测40例DLBCL，16例增生性淋巴结炎组织细胞中组蛋白H3、H4乙酰化水平、组蛋白H3K4、H3K9甲基化水平并进行分析和比较。结果在DLBCL组中，乙酰化组蛋白H3、H4和甲基化组蛋白H3K4阳性高表达显著低于增生性淋巴结炎组；而甲基化组蛋白H3K9的阳性高表达则显著高于增生性淋巴结炎组（P〈0．05），差异均有统计学意义。在DLBCL组，乙酰化组蛋白H3与乙酰化组蛋白H4、乙酰化组蛋白H3与甲基化组蛋白H3K4、乙酰化组蛋白H4与甲基化组蛋白H3K4的表达均有显著的相关性（P〈0．01）。结论DLBCL存在组蛋白乙酰化及甲基化调控异常，可能是致病的重要因素之一。

Objective To investigate the expression of histone acetylated H3 and H4, methylated H3K4 and H3K9 in diffuse large B-cell lymphoma （DLBCL）. Methods The expression of histone aeetylated H3 and H4, methylated H3K4 and H3K9 were examined by SP immunohistochemistry technique in lymphoid tissue of 40 cases with DLBCL and 16 cases with proliferative lymphadenitis. Results The expression of histone acetylation of H3 and H4 were lower than that in proliferative lymphadenitis. Histone methylated H3K4 was lower in expression and H3K9 was in higher expression. There was a positive correlative expression between the global histone acetylation of H3 and H4, the global histone acetylation of H3, H4 and histone methylation of H3K4. Conclusion Improper modification of histone aeetylations and methylations may play an important role in pathogenesis in DLBCL.