当归拈痛合剂的质量标准研究

Study on Quality Standard of Danggui Niantong Heji

目的：建立当归拈痛合剂的质量标准.方法：采用薄层层析法对当归拈痛合剂中的当归、苦参和甘草3味中药进行定性鉴别;用高效液相色谱（HPLC）法测定黄芩苷含量,色谱柱为Agilent公司Zorbax SB-C18（4.6×250mm）,流动相为甲醇-0.2%磷酸溶液（48∶52）;检测波长为278 nm,柱温为40℃,流速为1.0 mL/min.结果：在TLC中能够检出当归、苦参和甘草,阴性对照无干扰;黄芩苷进样量在0.25-2.50μg范围内与峰面积线性关系良好,平均加样回收率为101.8%,RSD为1.65%.结论：所用定性、定量方法简便、可靠、专属性强,重复性好,可用于当归拈痛合剂的质量控制.

Objective： To establish the quality standard of Danggui Niantong Heji. Methods： The TLC method for identification of Radix Angelicae Sinensis, Radix Sophorae Flavescentis, Radix et Rhizoma Glycyrrhizae were established. Baicalin was determined by HPLC. On Zorbax SB- C18 column（4.6× 250 mm）,the UV detector was set at 278 nm. The column temperature was at 40 ℃. The mobile phase was methanol-0.2%H3PO4 （48 ： 52） and the flow rate was 1.0 mL/min. Results： Radix Angelicae Sinensis, Radix Sophorae Flavescentis, Radix et Rhizoma Glycyrrhizae could be detected. The calibration curve showed good linearity. The average recovery rate of baicalin was 101.8% ,RSD=1. 65%. Conclusion The method is simple, rapid and reproducible. It is suitable for the quanlity control of Danggui Niantong Heji.