高效液相色谱法测定不同生长年限杜仲皮中桃叶珊瑚苷的含量

Quantitative Determination of Aucubin in Bark of Eucommia with Different Growth Years by HPLC

目的采用高效液相色谱法测定不同生长年限杜仲皮中桃叶珊瑚苷的含量。方法色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(10∶90),检测波长为203nm,流速1mL/min,柱温25℃。结果内皮中桃叶珊瑚苷含量以13年生杜仲最高,栓皮中桃叶珊瑚苷含量以30年生杜仲最高。仅从杜仲皮中桃叶珊瑚苷总含量考虑,30年左右为最佳采收期。但考虑到30年时间太长,且13,22,30年生杜仲中桃叶珊瑚苷的含量差别不大,最佳采收期可定为13年。结论所建立的杜仲皮中桃叶珊瑚苷含量测定方法重现性好、结果准确可靠,为杜仲皮的质量控制提供了参考。通过对不同生长年限杜仲皮中桃叶珊瑚苷含量的测定,为确定杜仲的最佳采收期提供了科学依据。

Objective To determine the content of aucubin in the bark of eucommia with different growth years by HPLC. Methods To adopt the Kromasil C18 column（250 mm ×4. 6 mm, 5 μm） with the mobile phase of MeOH- H2O （10： 90）, at the flow rate of 1 mL/min, the detection wavelength of 203 nm and at 25 ℃. Results The content of aueubin in the bark of 13- year eucommia was the highest. If only considering the total content of aucubin, the best harvest time of eueommia was 30 years. Considering 30- year growth was too long and the distinction of the content of aucubin in the bark of 13-, 22-, 30- year eucommia was not obvious, the best harvest time of eucommia could be 13 years. Conclusion This method to determine the content of aucubin is accurate and credible. The results provide the scientific basis for the rational harvest time of eucommia.