3,6'-二芥子酰基蔗糖抗抑郁作用及对新生大鼠海马神经前体细胞增殖的影响

Effect of 3,6'-Disinapoyl Sucrose on Proliferation of Neural Progenitor Cells from Neonatal Rat Hippocampus

目的探讨远志活性成分3,6'-二芥子酰基蔗糖(DISS)的抗抑郁作用及对新生大鼠海马神经前体细胞增殖的影响。方法通过小鼠悬尾和强迫游泳试验来考察DISS抗抑郁作用;体外分离培养新生大鼠海马神经前体细胞,同时给予DISS,用四甲基噻唑蓝(MTT)法测定细胞存活率,观察DISS对神经细胞增殖的影响,并初步探讨其抗抑郁的作用机制。结果DISS不同剂量(5,10,20mg/kg)给药后,能明显缩短小鼠在悬尾和强迫游泳试验中的不动时间,并且随着剂量的增大抗抑郁作用逐渐降低;DISS不同质量浓度(0.4,10,50μg/mL)对所培养具神经前体细胞特性的细胞有促进增殖的作用。结论DISS具有较好的抗抑郁作用,该作用可能是通过调控神经干细胞的增殖、促进功能性神经再生和保护作用实现的。

Objective To observe the antidepressant effect of bioactive compound 3, 6＇-disinapoyl sucrose（DISS） and its effect on the proliferation of neural progenitor cells. Methods The behavioral studies were performed in forced swimming test （FST） and tail suspension test （TST）. To further explore the mechanisms underlying their antidepressant- like activities, we used isolated and cultured neural progenitor cells. By using MTT assay, the effect of DISS on neural cells proliferation was observed. Results After a week of administration, DISS （5, 10, 20 mg/kg） could significantly reduce the duration of immobility in both FST and TST. DISS had the protective effect on increasing proliferation of hippocampus neural progenitor ceils. Conclusion The antidepressant- like effects of DISS and its mechanisms might be involved by upregulation of the progenitor cell proliferation of hippocampus.