高效液相色谱法测定肝康颗粒中甘草酸含量

Determination of Glycyrrhizic Acid in Gankang Granules by HPLC

目的建立测定肝康颗粒中甘草酸含量的高效液相色谱(HPLC)法。方法采用外标一点法,色谱柱为Diamonsil ODS1 C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.2mol/L醋酸铵-冰醋酸(67∶33∶1),流速为1.0mL/min,检测波长250nm。结果甘草酸进样量在0.5102～4.0820μg范围内与峰面积线性关系良好,回归方程Y=6422.014X-412.836,r=0.9996(n=5),平均加样回收率为99.29%,RSD为1.22%(n=6)。结论HPLC法简便、准确,专属性强,测定结果重现性好,可用于肝康颗粒中甘草酸的定量分析。

Objective To establish the method for determining the content of glycyrrhizic acid in Gankang Granules by HPLC. Methods The one point external standard method was used with Diamonsil ODS1 C18 column （250 mm × 4. 6 mm, 5 μm）, methanol -0. 2 mol/L ammonium acetate-glacial acetic acid（67：33： 1） as the mobile phase, the detection wavelength was 250 nm and the flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0. 510 2-4. 082 0 μg for the glycyrrhizic acid and the linear equation was Y= 6 422. 014X-412. 836, r = 0. 999 6（ n = 5）. The average recovery rate was 99.29% and RSD was 1.22% （n = 6）. Conclusion This method is simple and accurate with good specificity and reproducibility, which can be used for the scientific quantitative analysis of glycyrrhizic acid in Gankang Granules.