牙周病患者牙龈组织中PGE_2、6-K-PGF_(1a)和TXB_2定量分析及与牙周病的关系

TheRelationshipBetweenPGE2、6-K-PGF1aandTXB2inGingivawithPeriodontalDiseaseandPeriodontalDiseasesChenTielou,StomatologicalResearchCenterofNavy(200081);ZhouYijun,WuZhifen,StomatologicalColegeofFouthMilitoryMedicalUniversity(710032)AbstractAim:Tostudytherel

目的：探讨牙龈组织中前列腺素Ｅ２（ＰＧＥ２）、６－酮－前列腺素Ｆ１ａ和血栓素Ｂ２（ＴＸＢ２）与牙周病的关系．方法：选２０例牙周炎患者，１０例牙龈炎患者和１０例健康龈，分别用放免测定（ＲＩＡ）和免疫组化定量法对牙龈组织中ＰＧＥ２、６－Ｋ－ＰＧＦ１ａ和ＴＸＢ２定量．结果：牙周病患者牙龈组织中ＰＧＥ２含量最多，ＴＸＢ２次之，６－Ｋ－ＰＧＦ１ａ含量最少．牙周炎和牙龈炎组中的ＰＧＥ２、６－Ｋ－ＰＧＦ１ｄ和ＴＸＢ２含量与健康龈组比有显著性差异，牙周炎组中的ＰＧＥ２、６－Ｋ－ＰＧＦ１ａ和ＴＸＢ２量明显高于牙龈炎组．牙龈组织中的ＰＧＥ２、６－Ｋ－ＰＧＦ１ａ和ＴＸＢ２含量均从健康组、牙龈炎组到牙周炎组递增，免疫组化定量法对牙龈组织中前列腺素的测定结果与放免测定一致．结论：牙周病患者牙龈组织中的ＰＧＥ２、６－Ｋ－ＰＧＦ１ａ和ＴＸＢ２含量与牙周指数明显相关，前列腺素在牙周病发生中起重要作用。

Aim:To study the relationship between prostaglandin E 2(PGE 2)、6-Keto-Prostaglandin F la (6-K-PGF 1a and Thromboxane B 2(TXB 2) in gingiva with periodontal diseases.Methods:20 cases of peridontitis(P)、10 gingivitis(G) and 10 health(H) were selected,and measured the contents of PGE 2、6-K-PGF 1a and TXB 2 in gingiva of each patient by quantitative immunohistochemistry analysis and Radioimmunoassay(RIA).Results:The contents of PGE 2 in gingiva with periodontal diseases were more,TXB 2 were mediated,6-K-PGF 1a were less.There were more significant difference of PGE 2、6-K-PGF 1a and TXB 2 between group P or G and grap H.There were also more significont difference between group P and group G.The contents of PGE 2、6-K-PGF 1a and TXB 2 in gingiva were progressively incressed from H、G to P.The results of quantitative immunohistochemistry showed no difference with RIA,and this method can measure the contents of prostaglandins(PGs) in different layers and different cell to compensate RIA.Conclusion:The contents of PGE 2、6-K-PGF 1a and TXB 2 in gingiva with periodontal diseases were more interrelated to periodontal Index,and PGs play an immportant role in onset of periodontal disease,and combine RIA with quantitative immunohistochemistry can study the relationship between PGs and Periodontal disease better.