几个番茄品种AFLP指纹图谱的建立

Establishment of the AFLP fingerprinting for some tomato cultivars

应用优化的AFLP技术体系,构建了7份番茄黄化曲叶病毒病抗病材料和1份感病材料的指纹图谱。根据所得到的DNA指纹图谱数据,进行亲缘关系和遗传多样性分析。从64对引物中筛选出扩增效果稳定、多态性好的5对引物组合,分别对8份材料的基因组DNA进行扩增,共扩增出清晰的条带149条,其中62条带具有多态性,多态性位点百分率为41.6%。这一结果表明供试抗感黄化曲叶病毒病番茄材料在DNA水平上酶切位点的分布存在一定的差异。供试材料间的遗传相似系数的变化范围为0.83～0.95。以遗传相似系数0.89为阈值,进行UPGMA聚类分析,将8份番茄材料分成1个复合组和3个独立组。以上结果在DNA水平上为番茄抗黄化曲叶病毒病育种的亲本选配提供了参考依据。

The genetic relationship and diversity of 8 tomato germplasm resources which were resistant or suscepti- ble to tomato yellow leaf curl disease （TYLCD） were analyzed by the optimized AFLP （amplified fragments length polymorphism）. Five primer combinations selected from 64 primer pairs revealed a total number of 149 unambiguous bands,62 of which were polymorphic with a polymorphism frequency of 41.6%. This result showed molecular variations of some enzyme digestion sites among the used tomato germplasm resources. The genetic similarity coefficient of 8 tomato resources ranged from 0.83 to 0.95. Based on the coefficient value of 0.89,these tomato germplasm resources were clustered into 1 multiple-member group and 3 one-member groups by UPGMA （unweighted pair group method with arithmetic mean） analysis ,which provided molecular reference for parent selection in tomato breeding for resistance to TYLCD.