摘要
应用优化的AFLP技术体系,构建了7份番茄黄化曲叶病毒病抗病材料和1份感病材料的指纹图谱。根据所得到的DNA指纹图谱数据,进行亲缘关系和遗传多样性分析。从64对引物中筛选出扩增效果稳定、多态性好的5对引物组合,分别对8份材料的基因组DNA进行扩增,共扩增出清晰的条带149条,其中62条带具有多态性,多态性位点百分率为41.6%。这一结果表明供试抗感黄化曲叶病毒病番茄材料在DNA水平上酶切位点的分布存在一定的差异。供试材料间的遗传相似系数的变化范围为0.83~0.95。以遗传相似系数0.89为阈值,进行UPGMA聚类分析,将8份番茄材料分成1个复合组和3个独立组。以上结果在DNA水平上为番茄抗黄化曲叶病毒病育种的亲本选配提供了参考依据。
The genetic relationship and diversity of 8 tomato germplasm resources which were resistant or suscepti- ble to tomato yellow leaf curl disease (TYLCD) were analyzed by the optimized AFLP (amplified fragments length polymorphism). Five primer combinations selected from 64 primer pairs revealed a total number of 149 unambiguous bands,62 of which were polymorphic with a polymorphism frequency of 41.6%. This result showed molecular variations of some enzyme digestion sites among the used tomato germplasm resources. The genetic similarity coefficient of 8 tomato resources ranged from 0.83 to 0.95. Based on the coefficient value of 0.89,these tomato germplasm resources were clustered into 1 multiple-member group and 3 one-member groups by UPGMA (unweighted pair group method with arithmetic mean) analysis ,which provided molecular reference for parent selection in tomato breeding for resistance to TYLCD.
出处
《浙江农业学报》
CSCD
北大核心
2009年第5期424-428,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省科技计划项目(2007D70009)
农业科技成果转化资金项目(2007GB2C200129)
浙江省科技厅重大科技专项(2008C12002)
浙江省重大优先主题项目(2007C12003)