致敏血清干扰小鼠骨髓移植的作用研究

Effects of sensitized sera on bone marrow transplantation in a murine model

目的:研究致敏血清在小鼠骨髓移植中的作用,明确抗体介导的移植排斥作用。方法:在移植前1天将200μl致敏血清或非致敏血清注射到正常BALB/c小鼠中,移植受者经致死量照射后回输1×107 C57BL/6小鼠的骨髓细胞。应用补体依赖淋巴细胞毒性方法检测移植前受者的供者反应性抗体,并观察移植后受者的生存情况及植入分析。结果:致敏血清输注可促使供者反应性抗体存在于受者体内。骨髓移植后,接受致敏血清输注的受者80%于10天左右均死于骨髓衰竭,而接受非致敏血清输注的受者能长期存活。植入分析表明接受致敏血清输注的受者在移植后其血象及骨髓细胞随时间推移进行性下降,且供者细胞的嵌合百分比也进行性减少;与接受非致敏血清输注组相比,两组的差异有统计学意义(P<0.05)。结论:致敏血清能介导小鼠的骨髓移植排斥,其中供者反应性抗体介导的体液免疫发挥重要作用。

Objective： To investigate the effects of sensitized sera on bone marrow transplantation and clarify the role of antibody in the process of rejection. Methods： Two hundred microlitres sensitized sera or non-sensitized sera were injected into normal BALB/c one day before transplantation. Ten millions （1 ~ l0T） bone man^w cells from C57BIZ6 were transfused to the recipients after lethal irradiation. The donor-re- active antibodies in recipients before transplantation were measured by complement-dependent cytotoxicity （CDC） method. Moreover, the sur- vival analysis and engraftment evaluation of the recipients were carried post transplantation. Results：The CDC results showed that donor-reactive antibodies existed in the recipients which had received sensitized sera transfusion. Eighty percent （80%） of the recipients received sensitized sera transfusion died of bone marrow failure about 10 days post transplantation, while the recipients received non-sensitized sera transfusion were long-term alive. Furthermore, the hematopoietic recovery and percentage of donor chimera analysis declined along with time in the sensitized sera transfusion recipients, and there were significant differences compared with those in the non-sensitized sera transfusion recipients （P 〈 0.05）. Conclusion： Sensitized sera are capable of inducing rejection of bone marrow transplantation, and the donor-reactive antibodies play an important role in the process of rejection.