苦参素防治肝纤维化的实验研究

Experimental Study of Effect of Matrine on Liver Fibrosis

目的探讨苦参素注射液抗肝纤维化作用及对IL-10、TGF-β1和Ⅰ型胶原表达的影响,为临床应用中药防治肝纤维化和肝硬化提供实验依据。方法将35只SD大鼠随机分为对照组、CCl4诱导肝纤维化模型组和苦参素注射液干预组,检测Ⅰ型胶原在各组肝组织中的表达及IL-10、TGF-β1在各组血清中的表达。结果在正常肝组织、苦参素注射液干预组肝组织和肝纤维化模型组肝组织中Ⅰ型胶原表达的平均密度值分别为0.298,0.313,0.503,差异均有统计学意义(P<0.01);血清中IL-10表达含量分别为1412.38pg/L、1311.81pg/L、877.03pg/L,差异均有统计学意义(P<0.01),血清中TGF-β1表达含量分别为491.29pg/L、665.36pg/L、959.09pg/L,差异均有统计学意义(P<0.01)。结论苦参素可减轻CCl4诱导的肝纤维化,TGF-β1和IL-10的表达水平可作为评价临床上治疗肝纤维化的客观指标。

Objective To explore the therapeutic effect of matrine＇ on liver fibrosis and its influence on the expression of IL-10, TGF-β1 and type Ⅰ collagen in hopes of providing experiment data for the prevention and cure of liver fibrosis. Methods 35 SD rats were divided into normal control group, carbon tetrachloride （CCL4 ）-induced liver fibrosis model group and matrine intervention group randomly. Immunohistochemistry, HE and Masson staining were used to measure the expression of type Ⅰ collagen in liver tissue. The level of IL-10 and TGF-β1 in serum was measured by enzyme linked immunosorbent assay（ELLISA）. Results The average OD values of type Ⅰ collagen were 0. 298,0.313 and 0.503 in the normal control group,matrine group and liver fibrosis group,respectively. The level of IL-10 were 1 412.38 pg/L, 1 311.81 pg/L and 877.03 pg/L,the level of TGF-β1 were 491.29 pg/L, 665.36 pg/L and 959.09 pg/L, respectively. The difference had statistical significance between normal control group and liver fibrosis group, and between matrine group and liver fibrosis group （ P 〈 0. 01 ）. Conclusion In this CCl4-induced rat model,liver fibrosis was relieved by the administration of matrine. TGF-β1 and IL-10 may be use to evaluate the clinical intervention in the treatment of liver fibrosis.