摘要
慢病毒载体已经广泛应用于动物模型中基因治疗的研究和转基因动物的制备,而准确地测定重组慢病毒的滴度和感染效率是其关键步骤.通过荧光实时定量PCR的方法定量分析重组慢病毒的颗粒数以及病毒的活性滴度,并以GFP报告基因的方法作为对照来验证定量PCR方法的准确性.研究结果显示,应用荧光实时定量PCR法与GFP报告基因法测定得到的病毒活性滴度成正相关,而且前者可以更加准确地测定病毒滴度和病毒感染效率.
Lentiviral vector is being widely used in the study of gene therapy in animal models and in generating transgenic animals. However, determination of lentiviral particles and their infectivity is essential before their being used. Such a requirement can be accurately achieved by qRT-PCR. Refered by infectious units got from GFP reporter assay, it showed a positive correlation between the two approaches. A reliable, accurate and rapid method is therefore established for the determination of the recombinant lentiviral titer and the infectivity.
出处
《生命科学研究》
CAS
CSCD
2009年第5期394-398,共5页
Life Science Research
基金
国家高技术研究发展计划项目(2007AA021206)
国家自然科学基金资助项目(30870943)
上海市自然科学基金资助项目(08ZR1412100)
