苯乙酸钠处理的胃癌细胞对LAK细胞杀伤的敏感性

Increase in Lymphokineactivated Killer Sensitivity of Human Gastrocarcinoma Cell Lines After Sodium Phenylacetate Treatment

为了探讨分化诱导剂苯乙酸钠是否能够增强ＬＡＫ细胞的治疗效果，作者采用ＭＴＴ法分析了苯乙酸钠处理的胃癌细胞对ＬＡＫ细胞杀伤敏感性的变化。结果表明：苯乙酸钠处理的胃癌细胞ＭＫＮ－２８和ＭＫＮ－４５对ＬＡＫ细胞的杀伤敏感性均增强。另外，苯乙酸钠处理的胃癌细胞粘附ＬＡＫ细胞的活性亦增强。因此，苯乙酸钠处理的胃癌细胞对ＬＡＫ细胞杀伤敏感性的增强可能在于其增强了效靶细胞之间的识别和结合；苯乙酸钠与ＬＡＫ细胞相结合有可能增强ＬＡＫ细胞治疗肿瘤的效果。

The differentiation inducer of effect sodium phenylacetate on lymphokineactivated killer (LAK) sensitivity of human gastrocarcinoma cell lines (MKN28 & MKN45)was analyzed by MTT assay.The results showed that the LAK sensitivity of MKN28 and MKN45 cells was increased after sodium phenylacetate treatment. The sodium phenylacetate treatment also increased the binding rate of LAK cells to MKN28 and MKN45 cells. These data suggest that the effect of sodium phenylacetate in increasing LAK sensitivity of MKN28 and MKN45 cells may be due to its enhancement of recognizing and binding between effector and target cells. The efficacy of LAK therapy could be increased by combining LAK cells with differentiation inducer sodium phenylacetate.