宫颈鳞癌细胞中HPV和C-erbB-2基因扩增的检测

Detection of HPV and CerbB2 Amplification in Cervical squamous Cancer

应用ＰＣＲ和差别ＰＣＲ方法，平行检测４０例宫颈鳞癌和６例宫颈炎细胞中的ＨＰＶ感染和Ｃ－ｅｒｂＢ－２基因扩增。结果宫颈鳞癌中ＨＰＶ混合型别（ＨＰＶＳ）感染阳性率为６５．０％（２６／４０），其中，ＨＰＶ１６占３７．５％（１５／４０），ＨＰＶ１８仅占５．０％（２／４０）；而宫颈炎中仅１例ＨＰＶｓ感染，两者具有显著性差异（χ２＝５．０１，Ｐ＜０．０５），所有宫颈癌和宫颈炎中均不见Ｃ－ｅｒｂＢ－２基因扩增。结果表明：ＨＰＶ感染与宫颈鳞癌的发生关系密切，但与Ｃ－ｅｒｂＢ－２癌基因扩增没有直接联系。

Human papillomavirus and CerbB2 amplification were detected in 40 cases of cervical squamous carcinoma and 6 cases of cervicitis with PCR and DifferentialPCR methods, the positive rates of HPV mixedtypes, HPV16 and HPV18 were 65.0%,37.5%and 5.0%respectiely in cancer tissues, the positive rate of HPVs in cerviticitis was 16.7%, the difference were significant (χ2=5.01,P<0.05), but no CerbB2 amplification was found in both of them It shows that HPV has no directive effect on CerbB2 amplification in cervical squamous canncer.