低分子质量地黄多糖体外对Lewis肺癌细胞p53基因表达的影响

Effects of low-molecularweight rehmannia glutinosa polysaccharideson p53 gene expression in lewis lung cancer cells in vitro

目的研究低分子质量地黄多糖（ＬＲＰＳ）体外给药对Ｌｅｗｉｓ肺癌细胞ｐ５３基因表达的影响。方法利用竞争性逆转录聚合酶链反应（ＲＴ－ＰＣＲ）的方法检测ｐ５３基因表达的水平。结果ＬＲＰＳ在剂量０．１和０．５ｇ·Ｌ－１条件下，体外给药１２ｈ，Ｌｅｗｉｓ肺癌细胞内ｐ５３基因的表达水平为０．７９０和０．７１８，对照组为０．４８３，体外给药２４ｈ，ｐ５３基因的表达水平为０．９５７和０．５９０，对照组为０．２２６。结果表明，ＬＲＰＳ能使Ｌｅｗｉｓ肺癌细胞内的ｐ５３基因表达明显增加。结论ＬＲＰＳ可能通过调控ｐ５３基因的表达而影响肿瘤细胞的增殖、分化和凋亡。

AIM To study the effect of lowmolecularweight rehmannia glutinosa polysaccharides (LRPS) on p53 gene expresson in lewis lung cancer cells in vitro. METHODS The level of p53 gene expression was determined by competitive reverse transcription polymerase chain reaction (RTPCR). RESULTS The levels of p53 mRNA in lewis lung cancer cells were 0.483, 0.790 and 0.718 respectively after 12 h dealt with saline,LRPS 0.1 and 0.5 g·L^-1. They were 0.226,0.957 and 0.590 respectively after 24 h at the same condition. The experiment showed that p53 gene expression in cancer cells was markedly increased after LRPS. CONCLUSION LRPS may influence proliferation, differentiation and apoptosis of cancer cells by regulating p53 gene expression.