摘要
目的观察褪黑素拮抗氧自由基和对膜脂质过氧化损伤的保护作用。方法用电子自旋共振技术,以DMPO和4-POBN为捕集剂,捕集Fenton反应产生的羟自由基和Fe2+启动的鼠肝线粒体膜脂质过氧化产生的脂类自由基,以脂肪酸自旋标记物5-Doxyl和16-Doxyl标记线粒体膜,研究脂质过氧化损伤后膜流动性的变化。结果褪黑素对羟自由基和脂类自由基有良好的清除作用,50%清除浓度分别为186μmol·L-1和450μmol·L-1,而且在500μmol·L-1浓度范围内能防止脂质过氧化引起的线粒体膜流动性降低。结论褪黑素是一个有效的抗氧化剂,能防止生物膜的脂质过氧化损伤。
AIM To observe the effect of melatonins antagonism against oxygen free radical and protection biomembrane from lipid peroxidation damage. METHODS Using the ESR technique, DMPO and 4POBN as spintrapping traped hydroxyl radical in Fenton reaction and lipids radical generated from the lipid peroxidation of the mitochondrial membrane induced by ferrous. Lipidspecific spin labels 5Doxyl and 16Doxyl as lebeling probes lebeled mitochondrial membrane to study the change of mitochondrial membrane fluidity. RESULTS The results showed that melatonin has an effective scavenging effect on hydroxyl radical and lipids free radical. The IC50 was 186 μmol·L-1 and 450 μmol·L-1. Melatonin could prevent membrane fluidity decrease from lipid peroxidation damage. CONCLUTION Melatonin is an effective antioxidant which can protect biomembrane from lipid peroxidation damage.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第3期260-262,共3页
Chinese Pharmacological Bulletin
关键词
褪黑素
氧自由基
膜流动性
肝线粒体
melatonin
oxygen free radical
membrane fluidity
lipid peroxidation
ESR