小剂量茶碱对支气管哮喘患者外周淋巴细胞PAF受体mRNA表达的影响

Effects of small dose protheo on level of plateletactivating factor receptor mRNA expression in blood lymphatic cells of asthmatic patients

目的了解茶碱对支气管哮喘患者血小板活化因子（ＰＡＦ）受体ｍＲＮＡ表达的影响。方法采用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）技术检测了支气管哮喘患者及健康成人外周淋巴细胞ＰＡＦ受体ｍＲＮＡ的表达水平，探讨了小剂量茶碱对哮喘患者外周淋巴细胞ＰＡＦ受体ｍＲＮＡ表达的影响。结果（１）被检测的２０例健康成人中只有８例其外周淋巴细胞ＰＡＦ受体的ｃＤＮＡ可被扩增出来，而被检测的２１例哮喘患者均可获得ｃＤＮＡ片断；（２）服用小剂量茶碱１ｗｋ后，哮喘患者ＰＡＦ受体ｍＲＮＡ表达量（以“闪烁计数ｍｉｎ－１”表示，下同）与给药前的表达量相比无明显变化（Ｐ＞０．０５），服用２ｗｋ后，哮喘患者ＰＡＦ受体ｍＲＮＡ表达量由给药前的８６４０８±７４６２减少至６８９２７±５８８６（Ｐ＜０．０５），服用３ｗｋ后减少至５６７２０±４８６６（与给药前比较，Ｐ＜０．０１），接近健康人的表达量４９６８８±５４３８。结论连续服用小剂量茶碱可抑制外周淋巴细胞ＰＡＦ受体ｍＲＮＡ的表达。

AIM To study the effects of small dose protheo on level of plateletactivating factor (PAF) receptor mRNA expression in asthma blood lymphatic cells. METHODS To use reverse transcription of RNA followed by polymerase chain reaction (RTPCR) technique. RESULTS (1) cDNA region of PAF receptor in blood lymphatic cells was obtained from only 8 cases of 20 healthy adults but all 21 cases of asthmatic patients by RTPCR. (2) Small dose protheo (200 μg, three times a day) was used for asthma for one week, the level of PAF receptor mRNA expression in blood lymphatic cells showed no change vs before (P>005), this level was reduced for two weeks (P<005) and markedly reduced vs before (P<001) for three weeks, close to that of healthy adult. CONCLUSIONS Small dose protheo may inhibit PAF receptor mRNA expression in asthma blood lymphatic cells.