金荞麦提取物抑制肿瘤细胞侵袭、转移和HT-1080细胞产生Ⅳ型胶原酶的研究

Extract of fagopyrun cymosum meissn inhibits tumor cell invasion and metastasis and suppresses type Ⅳ collagenase production of HT1080 cells

目的探讨金荞麦提取物对肿瘤细胞侵袭和转移的影响。方法以人工重组基底膜及小鼠黑色素瘤高转移株自发性肺转移模型观察了金荞麦提取物对Ｂ１６－ＢＬ６细胞的体外抗侵袭活性和体内抗转移作用；用聚丙烯酰胺凝胶电泳法进一步观察了其对人纤维肉瘤ＨＴ－１０８０细胞Ⅳ型胶原酶的产生及活性的影响；同时用ＷＳＴ法观察了该药的细胞毒性。结果金荞麦提取物在１００ｍｇＬ－１剂量下能明显抑制Ｂ１６－ＢＬ６细胞侵袭；在２００ｍｇｋｇ－１剂量下能有效抑制Ｂ１６－ＢＬ６黑色素瘤细胞在Ｃ５７／ＢＬ６小鼠体内自发性肺转移。该药对Ｂ１６－ＢＬ６和ＨＴ－１０８０细胞无明显细胞毒作用。该药能抑制ＨＴ－１０８０细胞Ⅳ胶原酶的产生，但对酶的活性无明显影响。结论金荞麦提取物具有明显的抗癌侵袭和转移的作用。

AIM To study antiinvasive and antimetastatic activities of fagopyrun cymosum meissn on B16BL6 melanoma cells (highly metastatic cell line) and inhibitory effect on type Ⅳ collagenase production of HT1080 cells. METHODS Reconstituted basement membrane invasion assay was utilized to evaluate the invasive activity of cancer cells. Tyep Ⅳ collagenase production was assessed by PAGE substrate zymography. WST assay was used to examine the cytotoxicity of fagopyrun cymosum meissn. RESULTS The extract of fagopyrun cymosum meissn significantly inhibited membrane invasion of B16BL6 bearing C57/BL6 cell at a concentration of 100 mg·L-1 and effectively suppressed the spontaneous lung metastasis on B16BL6 bearing C57/BL6 mice at the dosage of 200 mg·kg-1. Extract of fagopyrun cymosum inhibited the production of type Ⅳ collagenases of human fibrosarcoma cells line HT1080 but it has no effect on the activity of this enzyme. CONCLUSION Extract of fagopyrun cymosum meissn has the notable inhibitory effects on tumor invasion and metastasis. Type Ⅳ collagenase inhibition may correlate with the antiinvasive and antimetastatic activities of Fagopyrun cymosum.