苯乙酸钠在体外对卵巢癌3AO细胞的生长抑制及其分化诱导

Growth inhibition and differentiation of ovarian cancer 3AO cells in vitro by sodium phenylacetate

目的研究非毒性分化诱导剂苯乙酸钠（ＮａＰＡ）在体外对卵巢癌３ＡＯ细胞的生长、分化以及表型的影响。方法以３Ｈ－ＴｄＲ参入法和半固体琼脂培养法分别检测了卵巢癌３ＡＯ细胞的着壁依赖性和着壁不依赖性生长。以酶联吸附试验（ＥＬＩＳＡ）检测了３ＡＯ细胞表面人白细胞抗原（ＨＬＡ）Ⅰ、Ⅱ类分子的表达。以电子显微镜观察了３ＡＯ细胞的超微结构。结果（１）ＮａＰＡ能够抑制３ＡＯ细胞的着壁依赖性和着壁不依赖性生长，而对正常羊膜组织Ｗｉｓｈ细胞的生长无显著抑制作用。（２）ＮａＰＡ能够增强３ＡＯ细胞表面ＨＬＡⅠ、Ⅱ类分子的表达。（３）以电子显微镜观察到：在ＮａＰＡ处理的３ＡＯ细胞中，线粒体、内质网以及弹力丝等丰富；而在ＮａＰＡ未处理的３ＡＯ细胞中，除了大量游离的多聚核糖体之外，上述的细胞结构几乎没有。结论ＮａＰＡ不仅能够选择性抑制卵巢癌细胞的生长，而且能够有效地诱导卵巢癌细胞的分化以及表型转变，为ＮａＰＡ分化治疗卵巢癌提供了实验依据。

AIM The effect of a nontoxic differentiation inducer, sodium phenylacetate (NaPA) on the growth, differentiation and phenotype of ovarian cancer 3AO cells in vitro was studied. METHODS The anchoragedependent and anchorageindependent growth of 3AO cells were evaluated using 3H thymidine incorporation assay and semisolid agar culture assay respectively. The expression of human leucocyte antigen (HLA) class Ⅰ and Ⅱ molecules on the surface of 3AO cells was measured using enzymelinked immunosorbent assay. The ultrastructure of 3AO cells was investigated by electron microscopy. RESULTS (1) NaPA treatment resulted in dosedependent inhibition of 3AO cell growth, including anchoragedependent and anchorageindependent growth. But NaPA was not significantly efficient to inhibitory to the growth of Wish cells from normal amnion tissue. (2) NaPA could enhance the expression of HLA class Ⅰ and Ⅱ molecules on the surface of 3AO cells. (3) Electron micrographs of NaPAtreated 3AO cells demonstrated the emergence of rich mitochondria, rough endoplasmic reticulum and elastic filament, in contrast to the scant beforementioned structures but numerous scattered polyribosomes in NaPAuntreated 3AO cells. CONCLUSION NaPA not only selectively inhibits the growth of ovarian cancer cells, but also is effective in inducing the differentiation and phenotypical modification of ovarian cancer cells. It suggests that NaPA may be potential in the differentiation therapy of ovarian cancer.