阿魏酸钠和维拉帕米对庆大霉素肾毒性的保护作用

Protective effects of sodium ferlate and verapamil on gentamicin nephrotoxicity

目的研究中药阿魏酸钠（ＳＦ）和维拉帕米（Ｖｅｒ）对庆大霉素（ＧＭ）肾毒性的保护作用。方法采用原代近端肾小管上皮细胞培养技术制作体外ＧＭ损伤模型，观察细胞丙氨酸氨基转移酶（ＡＬＴ）、过氧化氢酶（Ｃａｔ）、ＮＡＧ酶活性、ＤＮＡ合成，细胞内游离钙浓度以及形态学变化，同时观察ＳＦ、Ｖｅｒ的防治作用。结果ＧＭ组表现为ＡＬＴ、Ｃａｔ活性下降，ＮＡＧ活性增加。同时细胞ＤＮＡ合成下降，细胞内游离钙浓度升高。光镜发现近端小管上皮细胞有大量空泡和溶酶体髓样体形成。给予ＳＦ能使上述异常指标明显改善，组织学损害也明显减轻。Ｖｅｒ仅能保护ＡＬＴ活性和降低细胞内游离钙浓度。结论ＳＦ能有效保护ＧＭ对肾小管上皮细胞的损害。

AIM To investigate the effect of sodium ferulate (SF), a Chinese medicinal herb, and verapamil (Ver) on gentamicin (GM) nephrotoxicity in renal tubular cells. METHODS Alanine aminopeptidase (ALT), Catalase (Cat), Nacetylβglucosaminindase (NAG) activity, and DNA synthesis, sytosolic free calcium concentration, and morphological alterations were observed in gentamicininduced damage to cultured primary renal proximal tubular cells. The protection of SF and Ver to damages was observed too. RESULTS In GM group, ALT and Cat activity decreased, NAG activity increased. In addition, DNA synthesis decreased and cytosolic free calacium concentration increased. Electronmicroscope observation revealed an extensive vacuolization in proximal tubular cells and formation of myeloid bodies in lysosome. Simultaneous administration of SF with GM could markedly improve the values of parameters mentioned above and the histological damages, but Ver only could protect ALT activity and reduce cytosolic free calcium concentration. CONCLUSION SF can protect renal proximal tubular cell from GM nephrotoxicity.