摘要
目的优化大鼠C6脑胶质瘤模型的建立方法和观察大鼠C6脑胶质瘤生长规律的动态MRI影像,进行MRI影像学与病理学的对照研究。方法35只SD大鼠采用立体定向法建立脑胶质瘤模型,其中20只用于生存分析,余下15只随机分为三组,每组5只,分别于第7天,第14天和第21天行MRI动态观察并行瘤体体积测定。MRI影像学检查后,取脑行病理组织学和免疫组化检测。结果立体定向法建立大鼠C6胶质瘤模型成功率高,颅内接种成功率100%。大鼠接种后至第三周有大鼠开始出现死亡,荷瘤大鼠平均生存时间为28±1.5天。第一周末,第二周末,第三周末MRI扫描及瘤体积测定分别为:9.35±0.3 mm3,142.32±0.6 mm3,270.04±1.2 mm3。病理瘤体测量分别为:8.9±0.2 mm3,137.8±0.7 mm3,269.11±0.7 mm3。结论立体定向法是建立大鼠脑胶质瘤的有效方法,大鼠C6脑胶质瘤动物模型肿瘤生长具有一定规律,其生长特性和病理特征与人脑胶质瘤相似,可作为脑胶质瘤的基础和临床研究的理想模型。MRI能够反映大鼠C6脑胶质瘤的动态生长特征,为进一步胶质瘤的实验研究提供基础。
Objective Optimization of rat C6 glioma model in rats and observe the laws of C6 glioma growth dynamic MRI imaging,MRI imaging for the control study and pathology.Methods 35 SD rats using stereotactic brain glioma model was established,of which 20 for the survival analysis,and the remaining 15 were randomly divided into three groups,each group 5,respectively,in the first 7 days,14 days and the first 21 day trip to observe the dynamic parallel MRI determination of tumor volume.MRI imaging after brain line Histopathological and immunohistochemical detection.Results Stereotactic established C6 glioma model in rats a high success rate and success rate of intracranial inoculation 100%.Rats to the third week after inoculation of rats began to have killed and tumor-bearing rats with an average survival time was 28 ± 1.5 days.The first weekend,second weekend,the third weekend MRI scanning and determination of tumor size were：9.35±0.3 mm3,142.32±0.6 mm3,270.04±1.2 mm3.Pathological tumor measurements were：8.9±0.2 mm3,137.8±0.7 mm3,269.11±0.7 mm3.Conclusion Stereotactic brain is the establishment of an effective way to glioma,rat C6 glioma animal model of tumor growth with a certain regularity,and its growth characteristics and pathological features similar to human gliomas and as a glioma basic and clinical research of the ideal model.MRI can reflect the dynamic growth characteristics of rat C6 glioma experimental and provides a base for further research on brain glioma.
出处
《中国实验诊断学》
北大核心
2009年第10期1320-1323,共4页
Chinese Journal of Laboratory Diagnosis
基金
安徽省科技攻关项目(编号07010302202)
