人IL-5诱导的STAT3酪氨酸磷酸化

hIL-5 induces the tyrosine phosphorylation of STAT3

目的分析人早幼粒细胞白血病细胞内STAT3的酪氨酸磷酸化活化情况。方法培养人早幼粒细胞白血病细胞株HL-60,分别用浓度为0,1.0,10,100 ng/ml的人白细胞介素(hIL)-5刺激,然后利用特异性抗体,用免疫沉淀法、聚丙烯酰胺凝胶(SDS PAGE)电泳及Western Blot方法进行检测。结果检测到HL-60细胞内不同浓度的STAT3表达。结论一定浓度的人IL-5能同时诱导HL-60细胞内STAT3α和STAT3α的酪氨酸(Y705)磷酸化,且在一定范围内这种诱导作用与IL-5的浓度呈正相关。

Objective STAT3 protein is an important member of STAT family and it is activated mainly by the phosphorylation of tyrosine.This study is to analyse the expression of STAT3 in Promyelocytic leukemia cells.Methods Promyelocytic leukemia cell line HL-60 are cultured and stimulate with human IL（hIL） 5 in different concentration（0,1.0,10,100 ng/ml） then are detected with immunoprecipitation,SDS-PAGE and Western Blot.Result HL-60 cell line expresses tyrosine（Y705） phosphorylated STAT3α and STAT3β in different concentration.Conclusion hIL-5 induces tyrosine（Y705） phosphorylation of STAT3α and STAT3β in HL-60 cell line in a certain range of concentration and the tyrosine phosphorylation of STAT3 αand STAT3β induced by hIL-5 is dose depended.