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大鼠急性脊髓损伤中热休克蛋白47的Western Blot分析 被引量：1

Analysis of Western Blot of heat shock protein 47 in rat acute spinal cord injury
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摘要 目的研究大鼠急性脊髓损伤(SCI)中热休克蛋白47(HSP47)的表达情况。方法用Wistar大鼠制作钳夹型SCI模型,在SCI后3天及每周评价BBB评分,并在1周,3周,5周用Western Blot方法分析脊髓中HSP47的表达情况。结果正常Wistar大鼠脊髓组织低表达HSP47,在SCI后表达明显提高,且在第5周仍有上升趋势(P<0.01)。结论Wistar大鼠SCI后HSP47表达增加,HSP47可能参与到SCI的病理改变过程中。 Objective To study the changes of heat shock protein 47（HSP47） in rat acute spinal cord injury（SCI）.Methods A modle of clip-compression injury of spinal cord in rats was used.27 Wistar rats were divided to normal group,sham operation group and SCI group randomly.Basso,Beattie,Bresnahan（BBB） Locomotor Rating Scale were assessed at 3days and every weeks after SCI.They were sacrificed to study the expression level of HSP47 using Western Blot technique at 1,3 and 5 weeks.Results There was a lower expression level of HSP47 in normal group and sham operation group.But,the expression level of HSP47 after SCI was higher.The level of HSP47 probably ascended after 5 weeks.Conclusion There is a higher level of HSP47 after SCI in Wistar rats.HSP47 is probably involved in pathological change after SCI.
作者 何玉宝 杨晨 杨有庚 任宪盛
机构地区 吉林大学第二临床医学院骨科 吉林大学第一临床医学院骨关节科
出处 《中国实验诊断学》 北大核心 2009年第10期1352-1354,共3页 Chinese Journal of Laboratory Diagnosis
关键词 急性脊髓损伤 热休克蛋白47 钳夹型SCI模型 acute spinal cord injury heat shock protein 47 clip-compression injury of spinal cord
分类号 R651.2 [医药卫生—外科学]
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参考文献12

  • 1Xia Y, Zhao T, Li J, et al. Antisense vimentin cDNA combined with chondroitinase ABC reduces glial scar and cystic cavity formation following spinal cord injury in rats[J]. Biochem Biophys Res Commun,2008,377 (2) :562.
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同被引文献8

  • 1Xia Y, Zhao T, Li J, et al. Antisense vimentin cDNA combined with chondroitinase ABC reduces glial scar and cystic cavity formation following spinal cord injury in rats. Biochem Biophys Res Commun, 2008, 377 (2): 562-566.
  • 2Okada M, Miyamoto O, Shibuya S, et al. Expression and role of type Ⅰ collagen in a rat spinal cord contusion injury model. Neurosci Res, 2007, 58 (4): 371-377.
  • 3Taguchi T, Razzaque MS. The collagen-specific molecular chaperone HSP47: is there a role in fibrosis. Trends Mol Med, 2006, 13 (2): 45-53.
  • 4Kao CH, Chen SH, Chio CC, et al. Exogenous administration of glial cell line-derived neurotrophic factor improves recovery after spinal cord injury. Resuscitation, 2008, 77 (3): 395-400.
  • 5Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma, 1995, 12 (1): 1-21.
  • 6Oguro A, Sakurai T, Fujita Y, et al. The molecular chaperone HSP47 rapidly senses gravitational changes in myoblasts. Genes Cells, 2006, 11 ( 11 ): 1253-1265.
  • 7Razzaque MS, Taguchi T. Pulmonary fibrosis: cellular and molecular events. Pathol Int, 2003, 53 (3):133-145.
  • 8Ishida Y, Kubota H, Yamamoto A, et al. Type Ⅰ collagen in Hsp47- null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis. Mol Biol Cell, 2006, 17 (5): 2346-2355.

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中国实验诊断学
2009年 第10期

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