孕酮调节小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α的表达

Regulation of progesterone on interleukins and TNF-α expression of murine CD11c positive splenic dendritic cells

目的在mRNA水平上探讨性激素孕酮(Prog)对小鼠脾脏CD11c阳性树突状细胞(SDCs)合成的细胞因子IL-6I、L-10I、L-12和TNF-α的影响。方法采用免疫磁珠法分选小鼠CD11c阳性SDCs,用不同浓度的Prog处理SDCs 24h,采用RT-PCR在mRNA水平上检测SDCs合成细胞因子的变化。结果中浓度的Prog上调IL-6和IL-10的表达而高浓度则降低表达;各浓度组的Prog可上调细胞因子IL-12的水平而降低TNF-α的表达。结论Prog可能通过影响SDCs合成的细胞因子进而调控机体的免疫反应,低、中浓度的Prog使机体的免疫应答向Th2倾斜,而高浓度的Prog推动机体内的免疫反应向Th1型转变。

Objective To elucidate the effect of progesterone （Prog） on the eytokine expressions of murine CD11c positive splenic dendritic cells （SDCs）. Methods SDCs were purified by immune beads and treated with various concentrations of Prog for 24hours, then cytokine expressions of SDCs in the mRNA level were analyzed through reverse transcription-polymerase chain reaction （RT-PCR）. Results Prog increased the transcript of IL-6 in middle dose group but decreased it in high dose, and the level of IL-10 was increased in low and middle dose groups while decreased in high dose group. Furthermore, the transcripts of IL-12 and TNF-α were increased and decreased respectively in all Prog treated groups. Conclusion Prog might regulate organ immune response through influencing cytokine expressions of SDCs. Low- and middle-dose Prog switch organ immune response to Th2-type, while high-dose Prog develops a Th1-type immune response.