日本三角涡虫自发荧光组织结构的研究

Research on histological structure of autofluorescence of Dugesia japonica

目的研究涡虫自发荧光的组织结构,为其发育和再生生物学提供资料。方法采用HE、Masson和Van Gieson染色显示日本三角涡虫(Dugesia japonica)部分组织结构,并观察其紫外激发的自发荧光。每个染色组和自发荧光组有6条涡虫。结果其表皮、咽的外表皮、原肾管上皮、肠上皮、光感受器细胞和纵神经索都能自发蓝色荧光;交配囊上皮游离面可见自发黄绿色荧光,上皮中部发蓝色荧光,基底部发的蓝色荧光较弱;精巢几乎不发荧光,眼点的色素上皮不发荧光。结论对于涡虫眼点结构和自发荧光的研究可能会对无脊椎动物[的起源及系统]化规律的研究提供帮助。

Objective Hermaphroditic planarians possess a very important position in the systematic evolutionary history of animal, as they are capacity of complete regeneration. Hence, the research on histological structure of autofluorescence has been carried out to provide a crucial insight into the developmental and regenerative biology. Methods Part of histological structure of planarian （ Dugesia japonica ） was revealed with HE method, Masson method and Van Gieson method. Their autofluorescence was observed with ultraviolet. There were six planarians in each stained group and the autofluorescence group. Results Epidermis, outer epidermis of pharynx, protonephridium, intestine, the photoreceptor cells and longitudinal nerve cords, all radiated blue autofluorescence. The epithelial dissociation side of copulatory bursa radiated yellow autofluorescence, its middle part radiated blue autofluorescence, its fundus side radiated weakly blue autofluorescence. Testis could hardly give off autofluorescence. Pigment cells of eyepot could not give off autofluorescence. Conclusion The research on configuration and autofluorescence of planarian eye may offer help for the study of origin and evolutionary law on eye of invertebrate.