乳鼠心肌细胞移植对兔急性心肌梗死区微血管密度的影响

Effect of neonate rats' cardiomyocytes transplantation on myocardial capillary density of rabbits with acute myocardial infarction

目的观察乳鼠心肌细胞移植对兔急性心肌梗死区微血管密度及微血管再生的影响。方法新西兰大白兔16只,新生乳鼠40只。建立兔急性心肌梗死模型后,将培养的SD乳鼠心肌细胞移植到兔心肌梗死区,分别于移植后1、23、、4周取材,用碱性磷酸酶组织化学法和免疫荧光法进行染色,光镜观察,LEICA Qwin V3图像分析系统分析兔心肌梗死边缘区毛细血管密度及其再生情况。结果乳鼠心肌细胞移植后的1、2、3、4周不同的时间段,兔心肌梗死区的毛细血管密度及新生毛细血管数量均明显高于对照组(P<0.05)。定量分析显示,毛细血管密度呈现先升后降的趋势。结论异体异种心肌细胞移植能够促进兔心肌梗死区的毛细血管新生,有利于改善心肌的血液供应。

Objective To explore the influence of neonate rats＇ cardiomyocytes transplantation on myocardial capillary density and microvessel regeneration of rabbits with acute myocardial infarction（AMI）. Methods Sixteen New Zealand white rabbits and 40 neonate rats were used in the study. After the model of AMI in rabbits was established, cultured neonate rats＇ cardiomyocytes were injected into the area of AMI. Specimens were collected 1, 2, 3 and 4 weeks after transplantation, and they were stained by alkaline phosphatase （AIP） histochemical method and immunofluorescence method, then observed by light microscopy. The capillary density and regeneration around the marginal zone of AMI were assayed through LEICA Qwin V3 image analysis system. Results After neonate rats＇ cardiomyocytes transplantation for 1, 2, 3 and 4 weeks, the capillary density and the number of regenerate capillary around the area of rabbit＇ s AMI were increased significantly than those of the control group. And through quantitative analysis, we found that myocardial capillary density tend to be increased first and then decreased. Conclusion Allogenic cardiomyocytes transplantation could promote neovascularization of acute infarcted myocardium of rabbits, and then improve the blood supply of myocardium.