神经元型一氧化氮合酶和血管内皮生长因子在人胎早期小肠绒毛中的表达及意义

Expressions of nNOS and VEGF in the early developing small intestinal villi of human fetus and its significance

目的探讨神经元型一氧化氮合酶（nNOS）和血管内皮生长因子（VEGF）在人胎早期小肠绒毛中的表达规律。方法应用免疫组织化学SABC和Pv法检测第2、3、4月龄段，nNOS和VEGF在14例人胎小肠绒毛中的表达。结果第2月胚龄时，nNOS在小肠绒毛中呈阴性表达，VEGF在小肠绒毛上皮细胞呈阳性表达。第3-4月胎龄段，nNOS和VEGF在小肠绒毛部分细胞均呈阳性表达。结论nNOS和VEGF与人胎早期小肠绒毛的生长发育关系密切。

Objective To explore the rule of the expressions of neuronal nitric oxide synthase （nNOS） and vascular endothelial growth factor（VEGF） in the early developing human fetus. Methods At the second to fourth month of gestation, the expressions of nNOS and VEGF were observed in 14 cases of human fetal small intestine villi by using the SABC and PV of the immunohistoehemical methods. Results In the second month of gestation, the expression of nNOS was negative and VEGF was positive at the epithelial cell layer in the small intestinal villi of human fetus. At the third to fourth month of gestation, the expressions of nNOS and VEGF proteins were positive in some cells of the small intestinal villi of human fetus. Conclusion nNOS and VEGF proteins are closely related to the growth and development of the cells and tissues in the early developing small intestinal villi of human fetus.