两种显示大鼠不同组织微血管方法的比较

Comparison between two methods for displaying microvessels in rat different tissues

目的比较CD34免疫组织化学法和硝基四氮唑蓝/5-溴-4-氯-3-吲哚磷酸盐(NBT/BCIP)酶组织化学法显示不同组织微血管的特性。方法选取大鼠小肠、肾、脾、肝、心、肺和皮肤等7种组织,通过形态学分析和显微计数,分析上述两种方法的区别。结果CD34免疫组织化学法对所取各器官组织内微血管均能显示,NBT/BCIP酶组织化学法能显示心、肠、肾、肝组织内微血管,而不能显示皮肤、脾和肺组织内微血管;对同一组织同一切面,CD34免疫组织化学法显示微血管密度显著性低于NBT/BCIP酶组织化学法。结论CD34免疫组织化学法适于各种组织内微血管的显示,而NBT/BCIP酶组织化学法能够更大密度地显示部分器官组织内的微血管。

Objective To investigate the differences between CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry in displaying microvessels of various tissues. Methods The microvessels in rat small intestinal, kidney, spleen, liver, heart, lung and skin were stained by CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry, and the two methods were compared by morphological analysis and microscopic count. Results We found that the microvessels in all selected tissues above mentioned were stained by CD34 immunohistochemistry, and that the microvessels in small intestinal, kidney, liver and heart tissues, but not in spleen, lung and skin tissues, were stained by NBT/BCIP enzymic histochemistry. And in the same sections of one tissue, the microvascular density by NBT/BCIP enzymic histochemistry was higher than that by CD34 immunohistochemistry. Conclusion CD34 immunohistochemistry is generally suitable to display the microvesssels in various organs, while NBT/BCIP enzymic histochemistry shows some organ microvessels at higher density.