急性病毒感染对小鼠心肌糖蛋白130的基因及蛋白表达的影响

Effect of Acute Virus Infection on Expression of gp130 mRNA and Protein of Mice Myocyte

目的:探讨糖蛋白130(glycoprotein 130)在病毒性心肌炎中的作用。方法:建立病毒性心肌炎小鼠模型,观察其心肌病理和血清IL-6水平;用RT-PCR方法观察小鼠心肌gp130 mRNA的表达;Western blot方法观察gp130蛋白的表达。结果:病毒组与正常对照组相比,病毒组小鼠心肌病变检出率和血清IL-6水平显著高于对照组(P<0.001);心肌gp130 mRNA表达亦见明显增高(P<0.01),其蛋白表达未见明显改变(P>0.05)。结论:急性病毒感染促使小鼠心肌gp130基因表达,机制有待进一研究。

Objective： To study the role of glycoprotein （gp）130 in myocarditis. Methods：Thirty-two BALB/c mice were randomly divided into 2 groups, i.e. Coxsakievirus B3 （CVB3）infection group and control group. The myocardial injury of the heart biopsy samples and serum IL-6 levels were examined. The expression of gp130 mRNA and protein were observed by RT PCR and western blot, respectively. Results： Compared with those of controls, the rate of incidence and the extent of myocar- dium injury and serum /L-6 level of infected mice were significantly increased（P〈0. 001 ）, and the expression of gp130 mRNA were also increased（P〈0.01 ）, but the expression of gp130 protein were not changed significantly（P〉0.05）. Conclusion：CVB infection would increase the expression of gp130 gene in mice myocardium and the mechanism of such up-regulation needs fur ther exploration.