一株猪葡萄球菌的分离鉴定

Isolation and Identification of a Staphylococcus hyicus Strain

从患有渗出性皮炎的仔猪分离到1株革兰阳性球菌,生化试验、形态学观察进行初步鉴定,然后进行16 S rRNA基因片段的扩增和测序,将测序结果与GenBank上的已知序列进行同源性比较,结果与猪葡萄球菌的核酸序列同源性达到99.6%,综合可见该分离菌株为猪葡萄球菌,并将其命名为GDSH1。

A Gram-positive coccus strain was isolated the sick piglet suffered exudative epidermitis. Bio- chemistry identification, morphology observation were used to identify the bacterial strain,then 16 S rRNA gene fragment was amplified,sequenced,and compared with the known sequences from GenBank. The resuits showed it had 99.6% homology of nucleotide with Staphylococcus hyicus. It is concluded that the strain belongs to Staphylococcus hyicus, named GDSH1.