塔玛亚历山大藻的麻痹性贝毒研究

STUDIES ON PARALYTIC SHELLFISH POISONING OF ALEXANDRIUM TAMARENSE FROM HONG KONG WATERS

对暨南大学水生生物研究所于199芹年10月从香港海域底泥分离后于实验室人工培养的塔玛亚历山大藻运用高效液相色谱分析麻痹性贝毒的组成；按照美国分析化学家协会的小白鼠生物检定标准方法测试其毒性。结果表明，所含毒素成分主要是膝沟藻毒素-2（GTX2），含量为94.13×10－12g／cell;次要成分是膝沟藻毒素-4的N-磺基氨基甲酸衍生物C4，含量为15．67×10-12g／cell，测得其毒性为（3．23－4.11）×10－6MU／Cell。研究表明，所用的微藻麻痹性贝毒的提取方法和高效液相色谱分析方法都比较容易和有效。

The components and toxicity of paralytic shellfish poisoning (PSP) of Alexandrium tamarense isolated from Hong Kong waters in Oct. l99l and cultured in the laboratory in the Institute of Hydrobiology, Jinan University, were studied. The alga was collected by filtration and the PSP was extracted by O.O3mol / L CH3COOH .The PSP was analyzed by high performance liquid chromatography (HPLC) and mouse bioassay. The results show that the major toxic component from A. tamarense cultured is Gonyautoxin-2 (GTX-2) at a concentration of 94.l3xlO-12g /cell (87.8% of the total PSP toxin), and the minor component is N-sulfocarbamate derivative of GTX4 (C4) at a concentration of l5.67 x lO-12g / cell (l2.2% of the total PSP toxin). The toxicity was (3.23- 4.ll) X lO-6 M-U / cell by mouse bioassay according to the standard method of the Association of Official Analytical Chemists (AOAC). The presence of l% NaCl causes a decrease of around 3O% in the toxicity in mouse bioassay The cultad A. tamarense used in the present study appears to have a lower toxicity than that identified in previous studies elsewhere. The difference might be due to a combined effect of different culture conditions, sizes of the alga and the number of culturing passage. The methods of sample extration and HPLC analysis of PSP toxin used in the present study were efficient but simpler than those used by other investigators in the previous researeh.